CytoStream™ Mouse Anti-GPX1 Recombinant Antibody (VS13-YC463) (CAT#: VS13-YC463)
The CytoStream™ Mouse Anti-GPX1 Recombinant Antibody is specific for human GPX1. GPX1, a glutathione peroxidase, protects cells from oxidative damage, impacting cancer susceptibility and metabolic processes by regulating peroxidase activity. The anti-GPX1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44758/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44758/if_selected.jpg
Figure 2 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subset of germinal center and non-germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44758/124588_A_8_8_selected.jpg
(Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subset of germinal center and non-germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44758/124588_A_8_8_selected.jpg
Figure 3 IHC staining of human lung
(Immunohistochemical staining of human lung shows strong cytoplasmic positivity in macrophages.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11582/ihc_selected.jpg
(Immunohistochemical staining of human lung shows strong cytoplasmic positivity in macrophages.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11582/ihc_selected.jpg
Figure 4 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44758/124588_B_8_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44758/124588_B_8_5.jpg
Figure 5 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44758/124588_A_7_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44758/124588_A_7_3.jpg
Figure 6 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44758/124588_A_8_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44758/124588_A_8_5.jpg
Figure 7 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44758/124588_A_5_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44758/124588_A_5_6.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000233276-GPX1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000233276-GPX1
Specifications
- Immunogen
- Recombinant human glutathione peroxidase 1, aa 20-203.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human GPX1
- Species Reactivity
- Human
- Applications
- WB, IHC-P, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 50% Glycerol and 0.2% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Glutathione Peroxidase 1; Cellular Glutathione Peroxidase; EC 1.11.1.9; GSHPx-1; EC 1.11.1; GSHPX1; GPx-1; GPXD;
- Gene ID
- 2876
- UniProt ID
- P07203
- Long Name
- Glutathione Peroxidase 1
- Sequence Similarities
- Belongs to the glutathione peroxidase family.
- Cellular Localization
- Cytosol; Mitochondrion
- Protein Refseq
- NP_000572.2
- Function
- Protects the hemoglobin in erythrocytes from oxidative breakdown. In platelets, plays a crucial role of glutathione peroxidase in the arachidonic acid metabolism (PubMed:11115402).
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0760MZ | Recombinant Mouse Anti-Human GPX1 Antibody (clone 3B20) | ELISA | Mouse antibody |
| MRO-0676-CN | Rabbit Anti-GPX1 Recombinant Antibody (clone CBACN-247) | WB, IP | Rabbit IgG |
| MRO-0677-CN | Rabbit Anti-GPX1 Recombinant Antibody (clone JF0944) | WB, IF, IHC, IP | Rabbit IgG |
| VS3-CJ459 | Rabbit Anti-GPX1 Recombinant Antibody (VS3-CJ459) | WB, IP | Rabbit IgG |
| VS3-FY612 | Recombinant Rabbit Anti-GPX1 Antibody (clone R06-0H1) | WB, ICC, IF, IP | Rabbit IgG |
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