Rabbit Anti-GSTA4 Recombinant Antibody (clone R28-7G-8) (CAT#: VS3-FY2198)
This R28-7G-8 recombinant monoclonal antibody specifically recognizes human GSTA4 protein, a calculated weight of 26 kDa. It cross-reacts with rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R28-7G-8 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000).
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Normal Tissue
RNA Expression
Figure 1 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48934/119477_B_8_5.jpg
(Endothelial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48934/119477_B_8_5.jpg
Figure 2 RNA cell line category: Cell line enhanced (AF22, ASC TERT1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170899-GSTA4
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170899-GSTA4
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat GSTA4
- Species Reactivity
- Human, Rat
- Clone
- R28-7G-8
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 26kDa, while the observed molecular weight stands at 23kDa.
- Related Disease
- Degenerative diseases including Parkinson's disease, Alzheimer's disease, cataract formation, and atherosclerosis
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Glutathione S-Transferase Alpha 4; Glutathione S-Transferase A4-4; Glutathione S-Transferase A4; GST Class-Alpha Member 4; EC 2.5.1.18; S-(Hydroxyalkyl)Glutathione Lyase A4; Glutathione S-Aralkyltransferase A4
- Gene ID
- 2941
- UniProt ID
- O15217
- Sequence Similarities
- Belongs to the GST superfamily. Alpha family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Ubiquitination at Lys127 and Lys138 (NX_O15217 [NX_O15217-1])
Modification sites at PhosphoSitePlus (O15217)
- Protein Refseq
- XP_005249092.1; XP_011512836.1; XP_011512837.1; XP_054211236.1; XP_054211237.1
- Function
- Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This isozyme has a high catalytic efficiency with 4-hydroxyalkenals such as 4-hydroxynonenal (4-HNE).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2113z | Mouse Anti-GSTA4 Recombinant Antibody (clone 23H9) | WB, FC, IHC | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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