Recombinant Human Anti-H3N2 HA Antibody (H3v-47) (CAT#: PABC-404)

Figure 1 Prophylactic and therapeutic efficacy of mAb H3v-47 in mice.

The protective efficacy of mAbs was assessed by virus titers in lungs on 4 dpi (a, b). Dots in a, b indicate individual mice (n = 5 mice per group). The median titer is shown with the line, and the dotted line indicates the limit of detection (LOD).

Bangaru, S., Zhang, H., Gilchuk, I. M., Voss, T. G., Irving, R. P., Gilchuk, P., ... & Richt, J. A. (2018). A multifunctional human monoclonal neutralizing antibody that targets a unique conserved epitope on influenza HA. Nature communications, 9(1), 2669.

Figure 2 H3v-47 mAb exhibits ADCC activity.

a Cross-linking of FcγRlla. Binding curves were obtained by performing ELISA with serial dilutions of each antibody (H3v-47, H3v-12, or control mAb VRCO1 [to HIV]) onto HA-coated plates and assessing the ability of HA-bound mAbs to engage both Fc-binding sites on the soluble FcγRlla dimer. b Primary NK cell activation. Antibody (H3v-47, H3v-12, or VRC01) were added independently on 96-well plates coated with purified A/Sydnev/5/1997 H3 HA and incubated for 2h. The plates were washed, and 5 x 10⁵ purified NK cells were added to each well. The cells were stained with anti-human CD107a allophycocyanin-H7 Ab, anti-human CD3 PerCP, anti-human CD56 allophycocvanin, and anti-IFNγ AF700.

Bangaru, S., Zhang, H., Gilchuk, I. M., Voss, T. G., Irving, R. P., Gilchuk, P., ... & Richt, J. A. (2018). A multifunctional human monoclonal neutralizing antibody that targets a unique conserved epitope on influenza HA. Nature communications, 9(1), 2669.

Figure 3 Inhibition of egress of A/Minnesota/11/2010 H3N2v virus by IgG of mAbs H3v-47 or CR8020 or by the small molecule neuraminidase inhibitor zanamivir.

Dotted line represents virus titer in supernatant in the absence of antibody treatment. The experiment was conducted three times independently. The hemagglutination assay to determine virus titer was also conducted three times independently (n-3). The significance in the reduction of HA titer between H3v-47 and CR8020 was calculated at each concentration using 2-way ANOVA and displayed on the graph as ***(P< 0.001).

Bangaru, S., Zhang, H., Gilchuk, I. M., Voss, T. G., Irving, R. P., Gilchuk, P., ... & Richt, J. A. (2018). A multifunctional human monoclonal neutralizing antibody that targets a unique conserved epitope on influenza HA. Nature communications, 9(1), 2669.