Mouse Anti-HDAC3 Recombinant Antibody (VS3-CJ32)
CAT#: VS3-CJ32
This product is a mouse antibody that recognizes HDAC3.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52052/if_selected.jpg
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Specifications
- Immunogen
- Recombinant full length of human HDAC3
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human HDAC3
- Species Reactivity
- Human
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/mL (lot specific)
- Buffer
- 50% Glycerol, 1% BSA, PBS, pH7.4.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).
Target
- Alternative Names
- HD3; KDAC3; RPD3; RPD3-2
- Gene ID
- 8841
- UniProt ID
- O15379
- Sequence Similarities
- Belongs to the histone deacetylase family. HD type 1 subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Sumoylated in vitro.
- Protein Refseq
- NP_003874.2
- Function
- Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates (PubMed:23911289, PubMed:21030595, PubMed:21444723, PubMed:25301942, PubMed:28497810, PubMed:28167758).
Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed:23911289).
Histone deacetylases act via the formation of large multiprotein complexes (PubMed:23911289).
Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression (PubMed:23911289).
Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation (By similarity).
Contributes, together with XBP1 isoform 1, to the activation of NFE2L2-mediated HMOX1 transcription factor gene expression in a PI3K/mTORC2/Akt-dependent signaling pathway leading to endothelial cell (EC) survival under disturbed flow/oxidative stress (PubMed:25190803).
Regulates both the transcriptional activation and repression phases of the circadian clock in a deacetylase activity-independent manner (By similarity).
During the activation phase, promotes the accumulation of ubiquitinated ARNTL/BMAL1 at the E-boxes and during the repression phase, blocks FBXL3-mediated CRY1/2 ubiquitination and promotes the interaction of CRY1 and ARNTL/BMAL1 (By similarity).
The NCOR1-HDAC3 complex regulates the circadian expression of the core clock gene ARTNL/BMAL1 and the genes involved in lipid metabolism in the liver (By similarity).
Also functions as deacetylase for non-histone targets, such as KAT5, MEF2D, MAPK14 and RARA (PubMed:21030595, PubMed:21444723, PubMed:25301942, PubMed:28167758).
Serves as a corepressor of RARA, mediating its deacetylation and repression, leading to inhibition of RARE DNA element binding (PubMed:28167758).
In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response (PubMed:28167758).
In addition to protein deacetylase activity, also acts as protein-lysine deacylase by recognizing other acyl groups: catalyzes removal of (2E)-butenoyl (crotonyl) and 2-hydroxyisobutanoyl (2-hydroxyisobutyryl) acyl groups from lysine residues, leading to protein decrotonylation and de-2-hydroxyisobutyrylation, respectively (PubMed:28497810, PubMed:29192674, PubMed:34608293).
Catalyzes decrotonylation of MAPRE1 (PubMed:34608293).
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Related Diseases
Alcoholism
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "HDAC3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1575 | Rabbit Anti-HDAC3 Recombinant Antibody (clone DS1575AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0720-CN | Rabbit Anti-HDAC3 Recombinant Antibody (clone CBACN-259) | WB, IF, IHC, IP | Rabbit IgG |
| ZG-0411J | Mouse Anti-HDAC3 Recombinant Antibody (ZG-0411J) | WB | Mouse IgG |
| ZG-0412J | Mouse Anti-HDAC3 Recombinant Antibody (ZG-0412J) | WB, IHC, ELISA | Mouse IgG |
| ZG-0413J | Mouse Anti-HDAC3 Recombinant Antibody (ZG-0413J) | WB, IF, ELISA | Mouse IgG |
| ZG-0414J | Mouse Anti-HDAC3 Recombinant Antibody (ZG-0414J) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1001 | Anti-HDAC3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3097 | Anti-Human HDAC3 Immunohistochemistry Kit | IHC |
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