Mouse Anti-HIV-1 Gag Recombinant Antibody (clone p5F1) (CAT#: MRO-2859CQ)
Recombinant mouse antibody to Gag(329-350). p5F1 reacted with p24 from HIV-1 IIIB, Ada-M, 74V, and KM018 strains. p5F1 recognized peptide DC-22, Gag 329-350.
Specific Inquiry
WB
Figure 1 Western blotting analysis of the reactivity of three mAbs with the HIV-1 p24 protein.
HIV-1IIIB lysates transferred to nitrocellulose were detected with three mAbs or rabbit anti-rp24 serum by Western blotting.
Preparation and Characterization of Three Monoclonal Antibodies against HIV-1 p24 Capsid Protein
ELISA
Figure 2 Specificity of mAbs evaluated by modified sandwich ELISA.
Lysates of HIV-1IIIB, HIV-1Ada-M, HIV-174V, HIV-1KM018, SIVagmTYO-1 and SRV-1.
Preparation and Characterization of Three Monoclonal Antibodies against HIV-1 p24 Capsid Protein
DB
Figure 3 Epitope analysis by dot blot.
Diagram showed sites that each peptide or rp24 was applied to. Dot 1-7 represent GA-12, NI-15, DR-16, DC-22, PS-18, AG-23 and rp24 respectively.
Preparation and Characterization of Three Monoclonal Antibodies against HIV-1 p24 Capsid Protein
ELISA
Figure 4 HIV-1 p24 detection limit by modified sandwich ELISA.
Five-fold diluted HIV-1IIIB lysate, starting from 1:100.
Preparation and Characterization of Three Monoclonal Antibodies against HIV-1 p24 Capsid Protein
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Recognizes HIV-1 Gag(329-350)
- Species Reactivity
- HIV-1
- Clone
- p5F1
- Applications
- ELISA, WB, DB
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HIV-1 Gag antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Western Blot, Dot Blot.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone p5F1"
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MHC Tetramer for Virology
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2824CQ | Human Anti-HIV-1 Gag Recombinant Antibody (clone 91-6) | ELISA, RIA, WB | Human IgG1, λ |
| MRO-2825CQ | Human Anti-HIV-1 Gag Recombinant Antibody (clone 98-4.3) | ELISA, RIA, WB | Human IgG1, λ |
| MRO-2826CQ | Mouse Anti-HIV-1 Gag Recombinant Antibody (clone 3E11) | ELISA, WB | Mouse IgG1 |
| MRO-2827CQ | Mouse Anti-HIV-1 Gag Recombinant Antibody (clone 108/03) | ELISA, IF, WB, IP | Mouse IgG1 |
| MRO-2828CQ | Mouse Anti-HIV-1 Gag Recombinant Antibody (clone 10-E-7) | ELISA, WB, IF | Mouse IgG1 |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2824CQ-S(P) | Human Anti-HIV-1 Gag Recombinant Antibody (clone 91-6); scFv Fragment | ELISA, RIA, WB | Human scFv |
| MRO-2825CQ-S(P) | Human Anti-HIV-1 Gag Recombinant Antibody (clone 98-4.3); scFv Fragment | ELISA, RIA, WB | Human scFv |
| MRO-2826CQ-S(P) | Mouse Anti-HIV-1 Gag Recombinant Antibody (clone 3E11); scFv Fragment | ELISA, WB | Mouse scFv |
| MRO-2827CQ-S(P) | Mouse Anti-HIV-1 Gag Recombinant Antibody (clone 108/03); scFv Fragment | ELISA, IF, WB, IP | Mouse scFv |
| MRO-2828CQ-S(P) | Mouse Anti-HIV-1 Gag Recombinant Antibody (clone 10-E-7); scFv Fragment | ELISA, WB, IF | Mouse scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2832CQ-F(E) | Mouse Anti-HIV-1 Gag Recombinant Antibody (clone 11H9); Fab Fragment | ELISA, WB, IF | Mouse Fab |
| MRO-2833CQ-F(E) | Mouse Anti-HIV-1 Gag Recombinant Antibody (clone 15F8C7 (8C7)); Fab Fragment | WB | Mouse Fab |
| MRO-2834CQ-F(E) | Mouse Anti-HIV-1 Gag Recombinant Antibody (clone 1B2C12); Fab Fragment | WB | Mouse Fab |
| MRO-2835CQ-F(E) | Mouse Anti-HIV-1 Gag Recombinant Antibody (clone 1-B-7); Fab Fragment | ELISA, WB, IF | Mouse Fab |
| MRO-2836CQ-F(E) | Mouse Anti-HIV-1 Gag Recombinant Antibody (clone 1D9); Fab Fragment | ELISA, WB, IF | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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