Mouse Anti-HIV-1 Nef Recombinant Antibody (clone A7) (CAT#: MRO-4213CQ)
Recombinant mouse antibody to Nef(28-45). A7 can bind to the peptide spanning amino acids 28-45.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
ELISA
Figure 1 Recognition of Env proteins from different constructs by the selected scFv.
Binding of the two antibody classes scFv A7 and
A2 to gp140 and gp120 Env proteins determined by ELISA.
Trott, M., Weiß, S., Antoni, S., Koch, J., Von Briesen, H., Hust, M., & Dietrich, U. (2014). Functional characterization of two scFv-Fc antibodies from an HIV controller selected on soluble HIV-1 Env complexes: a neutralizing V3-and a trimer-specific gp41 antibody. PLoS One, 9(5).
ELISA
Figure 2 Analysis scFv-Fc A2 and A7 for autoreactivity against cardiolipin and phosphatidylserine.
Autoreactivity of purified scFvFc A2 and A7 was analyzed by commercial ELISA using the internal positive and negative control sera.
Trott, M., Weiß, S., Antoni, S., Koch, J., Von Briesen, H., Hust, M., & Dietrich, U. (2014). Functional characterization of two scFv-Fc antibodies from an HIV controller selected on soluble HIV-1 Env complexes: a neutralizing V3-and a trimer-specific gp41 antibody. PLoS One, 9(5).
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Recognizes HIV-1 Nef(28-45)
- Species Reactivity
- HIV-1
- Clone
- A7
- Applications
- ELISA, WB
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HIV-1 Nef antibody has been reported in applications of Enzyme-linked Immunosorbent Assay. ELISA: Detection was with anti c-myc antibody (300 ng/well) and HRP conjugated anti-mouse
antibody (1:1,000).
Target
- Alternative Names
- HIV Nef; Human Immunodeficiency Viruse Nef; Human Immunodeficiency Viruse; HIV Nef protein; HIV Nef-protein; p27; HIV1gp9; F-protein; Negative factor
- Gene ID
- 156110
- UniProt ID
- P04601
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-013 | Recombinant Anti-HIV nef VHH Single Domain Antibody | WB, ELISA, IP, FuncS | Llama VHH |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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