Rabbit Anti-HTA2 Recombinant Antibody (clone R08-1A5)
CAT#: VS3-FY1738
Clone R08-1A5 is a rabbit recombinant monoclonal antibody that detects human HTA2. It cross-reacts with rat. The histone H2A antibody is guaranteed for western blot analysis of histone H2A in K562, C6 lysates.
Tested Data
WB
Figure 1 Anti-HTA2 Antibody (VS3-FY1738) in WB.
The Histone H2A expression in K562, C6 lysates were detected using the anti-Histone H2A by western blot assay.
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Specifications
- Immunogen
- Synthetic peptides of histone H2A
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat HTA2
- Species Reactivity
- Human, Rat
- Clone
- R08-1A5
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 14 kDa, while the observed molecular weight stands at 14 kDa.
- Related Disease
- Liebenberg Syndrome and Anus Benign Neoplasm
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- H2A2
- Gene ID
- 852283
- UniProt ID
- P04912
- Sequence Similarities
- Belongs to the histone H2A family.
- Cellular Localization
- Chromosome, Nucleosome core, Nucleus
- Post Translation Modifications
- Phosphorylated to form H2AS128ph (gamma-H2A) in response to DNA double-strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks. Phosphorylation is dependent on the DNA damage checkpoint kinases MEC1/ATR and TEL1/ATM, spreads on either side of a detected DSB site and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. Gamma-H2A interacts with ARP4, a shared component of the NuA4 histone acetyltransferase complex and the INO80 and SWR1 chromatin remodeling complexes, and serves to recruit first NuA4, mediating histone H4 acetylation, and subsequently the INO80/SWR1 complexes, facilitating DNA resection, to DSB sites. Gamma-H2A is required for sequestering cohesin around the break site, which is important for efficient post-replicative double-strand break repair by homologous recombination, holding the damaged chromatid close to its undamaged sister template. Gamma-H2A is removed from the DNA prior to the strand invasion-primer extension step of the repair process and subsequently dephosphorylated by PPH3, a component of the histone H2A phosphatase complex (HTP-C). Dephosphorylation is necessary for efficient recovery from the DNA damage checkpoint.
- Function
- Core component of nucleosome which plays a central role in DNA double strand break (DSB) repair. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "HTA2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK2055 | Rabbit Anti-HTA2 (pS129) Recombinant Antibody (clone R63-7J-2) | WB | Rabbit IgG |
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