Anti-ID3 Antibody Prodrug, Protease Activated (BCH-4/#3-3)
CAT#: VS-1025-YC72
The Anti-ID3 Antibody Prodrug, Protease Activated (BCH-4/#3-3) is an antibody prodrug comprised of a recombinant rabbit antibody targeting human ID3, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to ID3 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to ID3 effectively. ID3 (Inhibitor of DNA binding 3) is a helix-loop-helix transcription factor that acts as a tumor suppressor by blocking the activity of pro-proliferative factors. Its loss-of-function mutations or deletions are frequent in certain aggressive lymphomas (like Burkitt lymphoma) and B-ALL. The loss of ID3 removes a key regulatory brake on cell proliferation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21353/613_G9_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows strong nuclear positivity in bone marrow poietic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24677/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human gall bladder
Immunohistochemical staining of human gall bladder shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21353/47677_A_6_4_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24677/53711_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24677/53711_A_9_3.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24677/53711_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24677/53711_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (U-698)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117318-ID3
❮
❯
Specifications
- Host Animal
- Rabbit
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human ID3
- Target
- ID3
- Species Reactivity
- Human
- Isotype
- IgG
- Clone
- BCH-4/#3-3
- Affinity Constant
- KD = 22.4 pM
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- ID3 is a helix-loop-helix (HLH) protein that can form heterodimers with other HLH proteins. However, the ID3 lacks a basic DNA-binding domain and therefore inhibits the DNA binding of any HLH protein with which it interacts.
- Alternative Names
- HEIR-1; bHLHb25; ID-like protein inhibitor HLH 1R21; helix-loop-helix protein HEIR-1
- Gene ID
- 3399
- UniProt ID
- Q02535
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone BCH-4/#3-3"
- CAT
- Product Name
See other products for "ID3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2316MZ | Recombinant Mouse Anti-Human ID3 Antibody | ICC, IF, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0278-YJ | Rabbit Anti-ID3 Recombinant Antibody (clone BCH-4/#3-3) | ELISA, WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0279-YJ | Rabbit Anti-ID3 Recombinant Antibody (clone BCH-4/#16-1) | ELISA, WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0280-YJ | Rabbit Anti-ID3 Recombinant Antibody (clone BCH-4/#17-3) | ELISA, WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0278-YJ-S(P) | Rabbit Anti-ID3 Recombinant Antibody (clone BCH-4/#3-3); scFv Fragment | ELISA, WB, IHC | Rabbit scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0279-YJ-S(P) | Rabbit Anti-ID3 Recombinant Antibody (clone BCH-4/#16-1); scFv Fragment | ELISA, WB, IHC | Rabbit scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0280-YJ-S(P) | Rabbit Anti-ID3 Recombinant Antibody (clone BCH-4/#17-3); scFv Fragment | ELISA, WB, IHC | Rabbit scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0278-YJ-F(E) | Rabbit Anti-ID3 Recombinant Antibody (clone BCH-4/#3-3); Fab Fragment | ELISA, WB, IHC | Rabbit Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0279-YJ-F(E) | Rabbit Anti-ID3 Recombinant Antibody (clone BCH-4/#16-1); Fab Fragment | ELISA, WB, IHC | Rabbit Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0280-YJ-F(E) | Rabbit Anti-ID3 Recombinant Antibody (clone BCH-4/#17-3); Fab Fragment | ELISA, WB, IHC | Rabbit Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY720 | Recombinant Mouse Anti-ID3 Antibody (clone 4C8D8) | ELISA, IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3379 | Anti-ID3 Immunohistochemistry Kit | IHC |
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