Mouse Anti-IGFBP1 Recombinant Antibody (clone 1E1B2)
CAT#: ZG-0202U
Mouse anti-IGFBP1 antibody (clone 1E1B2) displayed high specificity against the IGFBP1 protein of Human and Mouse.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-IGFBP1 Antibody (ZG-0202U) in WB
All lanes: Mouse anti- Human Insulin-like growth factor-bindi ng protein 1 monoclonal antibody at 1μg/ml
Lane 1: Pyrolysis liquid human placental tissue 7.5μg/ml
Lane 2: Pyrolysis liquid human placental tissue 3.25μg/ml
Lane 3: IGFBP1 transfected SF9 cell lysate
Lane 4: human amniotic fluid
Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size : 28kd
Observed band size : 35kd
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Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46972/1334_E3_6_selected.jpg
Normal Tissue
Figure 2 Kidney
Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46972/146497_A_9_5.jpg
Normal Tissue
Figure 3 High expression in placenta
Decidual cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4445/146491_A_3_7.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (Hep G2, U-2197, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000146678-IGFBP1
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Specifications
- Immunogen
- Recombinant Human IGFBP1 Protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human IGFBP1
- Species Reactivity
- Human, Mouse
- Clone
- 1E1B2
- Applications
- ELISA, WB
- Conjugate
- Unconjugated
- Related Disease
- Ovarian Disease and Hyperinsulinism
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 0.1 M NaCl and 15 mM NaN3.
- Preservative
- 15mM NaN3
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot. Specific conditions should be optimized by the end user.
WB:1:500-1:5000
Target
- Alternative Names
- AFBP; IBP1; PP12; IGF-BP25; hIGFBP-1; insulin-like growth factor-binding protein 1; IBP-1; IGF-binding protein 1; IGFBP-1; alpha-pregnancy-associated endometrial globulin; amniotic fluid binding protein; binding protein-25; binding protein-26; binding protein-28; growth hormone independent-binding protein; placental protein 12
- Gene ID
- 3484
- UniProt ID
- P08833
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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