Rabbit Anti-IL18BP Recombinant Antibody (clone R31-4Z-6) (CAT#: VS3-FY2188)
Clone R31-4Z-6 is a rabbit recombinant monoclonal antibody that detects human IL18BP. It cross-reacts with rat. For detection of human IL18BP using WB and IHC, please refer to clone R31-4Z-6. Western blot demonstrated that R31-4Z-6 recognized a target protein with a molecular weight of 21 kDa.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25980/663_F4_4_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25980/663_F4_4_selected.jpg
Figure 2 Cerebral cortex
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41061/137724_B_8_5.jpg
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41061/137724_B_8_5.jpg
Figure 3 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41061/137724_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41061/137724_A_8_5.jpg
Figure 4 Testis
(Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41061/137724_A_6_6.jpg
(Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41061/137724_A_6_6.jpg
Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000137496-IL18BP
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000137496-IL18BP
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat IL18BP
- Species Reactivity
- Human, Rat
- Clone
- R31-4Z-6
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 21kDa, while the observed molecular weight stands at 40kDa.
- Related Disease
- Crohn's disease
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Target
- Alternative Names
- Interleukin 18 Binding Protein
- Gene ID
- 10068
- UniProt ID
- O95998
- Cellular Localization
- Secreted
- Post Translation Modifications
- N- and O-glycosylated.
O-glycosylated with core 1-like and core 2-like glycans.
O-glycan heterogeneity at Ser-53: HexHexNAc (major) and Hex2HexNAc2 (minor).
N-glycan heterogeneity at Asn-103: Hex5HexNAc4 (minor), dHex1Hex5HexNAc4 (major) and Hex6HexNAc5 (minor); N-glycan at Asn-147: dHex1Hex5HexNAc4.
(O95998-I18BP_HUMAN)
Glycosylation at Ser46, Thr47, Ser53, Asn79, Asn94, Asn103, and Asn147 (NX_O95998 [NX_O95998-2])
Modification sites at PhosphoSitePlus (O95998)
Glycosylation from GlyConnect I18BP_HUMAN (669)
Glycosylation from GlyGen (O95998) 8 sites, 6 N-linked glycans (2 sites), 6 O-linked glycans (4 sites)
- Protein Refseq
- XP_016872548.1; XP_016872550.1; XP_016872552.1; XP_024304071.1; XP_024304072.1
- Function
- Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "IL18BP"
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-205CT | Mouse Anti-IL18BP Recombinant Antibody (TAB-205CT) | ELISA | Mouse IgG |
| TAB-206CT | Mouse Anti-IL18BP Recombinant Antibody (TAB-206CT) | ELISA | Mouse IgG |
| TAB-205CT-S(P) | Mouse Anti-IL18BP Recombinant Antibody; scFv Fragment (TAB-205CT-S(P)) | ELISA | Mouse scFv |
| TAB-206CT-S(P) | Mouse Anti-IL18BP Recombinant Antibody; scFv Fragment (TAB-206CT-S(P)) | ELISA | Mouse scFv |
| TAB-205CT-F(E) | Mouse Anti-IL18BP Recombinant Antibody; Fab Fragment (TAB-205CT-F(E)) | ELISA | Mouse Fab |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0233CT | Recombinant Mouse anti-Human IL18BP Monoclonal antibody (NN0480-20H47) | WB | |
| VS3-QX593 | Mouse Anti-IL18BP Recombinant Antibody (clone 5C7-D2-G4-B6) | WB | Mouse IgG2b |
| VS4-WK249 | Mouse Anti-IL18BP Recombinant Antibody (VS4-WK249) | ELISA, WB | Mouse IgG |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1244CQ | Mouse Anti-IL18BP Recombinant Antibody (clone CBL078) | Neut, WB | Mouse IgG1 |
| NEUT-1245CQ | Mouse Anti-IL18BP Recombinant Antibody (clone CBL545) | Neut | Mouse IgG1 |
| NEUT-1246CQ | Mouse Anti-IL18BP Recombinant Antibody (clone 10G36) | Neut, WB | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1795 | Rabbit Anti-IL18BP Recombinant Antibody (clone DS1795AB) | ELISA, WB | Rabbit IgG |
| MOR-4249 | Rabbit Anti-IL18BP Recombinant Antibody (clone SI328DS) | ELISA | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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