Rabbit Anti-ING1 Recombinant Antibody (clone R03-6B7)
CAT#: VS3-FY2016
The R03-6B7 recombinant monoclonal antibody specifically attaches to human ING1. It cross-reacts with mouse, rat. This ING1 antibody is guaranteed for western blot analysis of ING1 in C6, Raw264.7 lysates and western blot analysis of ING1 in Hela, A549, HL-60, U251, U87-MG lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52591/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human thyroid gland
Immunohistochemical staining of human thyroid gland shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16136/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human gall bladder
Immunohistochemical staining of human gall bladder shows nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17773/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16136/38694_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16136/38694_A_9_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16136/38694_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16136/38694_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16136/38694_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000153487-ING1
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WB
Figure 1 Anti-ING1 Antibody (VS3-FY2016) in WB.
The ING1 expression in C6, Raw264.7 lysates were detected using the anti-ING1 by western blot assay.
WB
Figure 2 Anti-ING1 Antibody (VS3-FY2016) in WB.
The ING1 expression in Hela, A549, HL-60, U251, U87-MG lysates were detected using the anti-ING1 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human ING1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ING1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R03-6B7
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 47 kDa, while the observed molecular weight stands at 47 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Inhibitor Of Growth Family Member 1
- Gene ID
- 3621
- UniProt ID
- Q9UK53
- Sequence Similarities
- Belongs to the ING family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q9UK53)
Modification sites at neXtProt (NX_Q9UK53)
Glycosylation from GlyGen (Q9UK53) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001254657.1; NP_005528.4; NP_937860.1; NP_937861.1; NP_937862.1
- Function
- Cooperates with p53/TP53 in the negative regulatory pathway of cell growth by modulating p53-dependent transcriptional activation. Implicated as a tumor suppressor gene.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ING1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1765z | Mouse Anti-ING1 Recombinant Antibody (clone 41G11) | WB, ELISA, IF, IP | Mouse IgG1, κ |
| MOB-1194MZ | Recombinant Mouse Anti-Human ING1 Antibody (clone 3E7) | WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1829 | Rabbit Anti-ING1 Recombinant Antibody (clone DS1829AB) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3556 | Anti-ING1 Immunohistochemistry Kit | IHC |
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