Rabbit Anti-IRF8 Recombinant Antibody (clone R07-8I1)
CAT#: VS3-FY1751
This antibody was purified by affinity chromatography. It is a rabbit recombinant monoclonal antibody that exhibits high specificity and affinity for human IRF8. The R07-8I1 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunoprecipitation (dilution 1/20).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2267/1030_A12_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line THP-1
Immunofluorescent staining of human cell line THP-1 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2531/1855_B8_33_selected.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13480/33203_A_5_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2267/8046_A_8_8.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (Daudi, THP-1, U-698)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000140968-IRF8
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Specifications
- Immunogen
- Recombinant human IRF8 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human IRF8
- Species Reactivity
- Human
- Clone
- R07-8I1
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 48 kDa, while the observed molecular weight stands at 48 kDa.
- Related Disease
- Immunodeficiency 32A and Immunodeficiency 32B
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Interferon Regulatory Factor 8
- Gene ID
- 3394
- UniProt ID
- Q02556
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Ubiquitinated (PubMed:25122610).
Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes (By similarity).
Ubiquitination leads to its degradation by the proteasome (PubMed:25122610).
- Protein Refseq
- XP_047290008.1; NP_001350836.1; NP_001350837.1; NP_002154.1
- Function
- Plays a negative regulatory role in cells of the immune system (By similarity).
Involved in CD8⁺ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (By similarity).
Required for the development of plasmacytoid dendritic cells (pDCs), which produce most of the type I IFN in response to viral infection (By similarity).
Positively regulates macroautophagy in dendritic cells.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "IRF8"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1002MZ | Recombinant Mouse Anti-Human IRF8 Antibody (clone nBcdbn72860) | IHC-P | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3597 | Anti-IRF8 Immunohistochemistry Kit | IHC |
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