Mouse Anti-KIF11 Recombinant Antibody (VS3-CJ21)
CAT#: VS3-CJ21
This product is a mouse antibody that recognizes KIF11.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10093/1401_H1_6_selected.jpg
Normal Tissue
Figure 2 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10568/28948_A_9_8.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10568/28948_B_8_5.jpg
Normal Tissue
Figure 4 Oral mucosa
Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10568/28948_A_7_1.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10568/28948_A_8_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10568/28948_A_8_4.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138160-KIF11
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Specifications
- Immunogen
- Recombinant fragment of human KIF11 (N-terminus)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human KIF11
- Species Reactivity
- Human
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/mL (lot specific)
- Buffer
- 50% Glycerol, 1% BSA, PBS, pH7.4.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).
Target
- Alternative Names
- EG5; HKSP; KNSL1; MCLMR; TRIP5
- Gene ID
- 3832
- UniProt ID
- P52732
- Sequence Similarities
- Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. BimC subfamily.
- Cellular Localization
- Cytoplasm, Cytoskeleton, Microtubule
- Post Translation Modifications
- Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase).
A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.
- Protein Refseq
- NP_004514.2
- Function
- Motor protein required for establishing a bipolar spindle during mitosis (PubMed:19001501).
Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface (PubMed:23857769).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "KIF11"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3056z | Mouse Anti-KIF11 Recombinant Antibody (clone 23G5) | WB, ELISA, ICC | Mouse IgG1 |
| MRO-0520-CN | Recombinant Rabbit Anti-KIF11 Monoclonal Antibody (JU83-16) | WB, IP | Rabbit IgG |
| MRO-1833-CN | Rabbit Anti-KIF11 Polyclonal Antibody (MRO-1833-CN) | IF, IHC, FC | Rabbit IgG |
| VS3-QX636 | Mouse Anti-KIF11 Recombinant Antibody (clone 4H3-1F12) | WB, IP, ICC | Mouse IgG1 |
| VS3-FY2750 | Mouse Anti-KIF11 Recombinant Antibody (VS3-FY2750) | WB, IHC, FC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1934 | Hi-Affi™ Recombinant Rabbit Anti-KIF11 Monoclonal Antibody (DS1934AB) | ICC, IF, IP, WB | IgG |
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