Rabbit Anti-KIFAP3 Recombinant Antibody (clone R03-6C6)
CAT#: VS3-FY2018
Clone R03-6C6 is a rabbit recombinant monoclonal antibody that firmly attaches to human KIFAP3. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IHC-P. The KIFAP3 antibody has been teted positive by western blot analysis of KIFAP3 in A549, HL-60, C6 lysates and immunohistochemistry analysis of paraffin-embedded human lung cancer.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to microtubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23742/236_H10_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows strong nuclear and cytoplasmic positivity in cortical cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23738/ihc_selected.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23738/52632_A_7_8.jpg
Normal Tissue
Figure 4 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23738/52754_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23738/52632_A_9_3.jpg
Normal Tissue
Figure 6 Heart muscle
Cardiomyocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23738/52754_B_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000075945-KIFAP3
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WB
Figure 1 Anti-KIFAP3 Antibody (VS3-FY2018) in WB.
The KIFAP3 expression in A549, HL-60, C6 lysates were detected using the anti-KIFAP3 by western blot assay.
IHC
Figure 2 Anti-KIFAP3 Antibody (VS3-FY2018) in IHC.
Immunohistochemistry staining of paraffin-embedded human lung cancer using the anti-KIFAP3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide of human KIFAP3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat KIFAP3
- Species Reactivity
- Human, Rat
- Clone
- R03-6C6
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 91 kDa, while the observed molecular weight stands at 91 kDa.
- Related Disease
- Human lung cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Kinesin Associated Protein 3; Kinesin-Associated Protein 3; Smg GDS-Associated Protein; KAP-3; SMAP; KAP3; Small G Protein GDP Dissociation Stimulator
- Gene ID
- 22920
- UniProt ID
- Q92845
- Post Translation Modifications
- Phosphorylated on tyrosine residues by SRC in vitro; this reduces the binding affinity of the protein for RAP1GDS1. (Q92845-KIFA3_HUMAN)
Ubiquitination at Lys59, Lys469, Lys477, and Lys485 (NX_Q92845 [NX_Q92845-1])
Modification sites at PhosphoSitePlus (Q92845)
Glycosylation from GlyGen (Q92845) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_024309954.1; XP_047305487.1; XP_047305488.1; XP_047305493.1; XP_054191187.1
- Function
- Involved in tethering the chromosomes to the spindle pole and in chromosome movement. Binds to the tail domain of the KIF3A/KIF3B heterodimer to form a heterotrimeric KIF3 complex and may regulate the membrane binding of this complex (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| MOR-1942 | Hi-Affi™ Recombinant Rabbit Anti-KIFAP3 Monoclonal Antibody (DS1942AB) | WB, IHC-P, ICC | IgG |
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| VS4-WK268 | Rabbit Anti-KIFAP3 Recombinant Antibody (clone 28E9) | ELISA, WB, IHC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| VS-0525-XY3780 | Anti-KIFAP3 Immunohistochemistry Kit | IHC | |
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