Rabbit Anti-KIFAP3 Recombinant Antibody (clone R03-6C6) (CAT#: VS3-FY2018)
Clone R03-6C6 is a rabbit recombinant monoclonal antibody that firmly attaches to human KIFAP3. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IHC-P. The KIFAP3 antibody has been teted positive by western blot analysis of KIFAP3 in A549, HL-60, C6 lysates and immunohistochemistry analysis of paraffin-embedded human lung cancer.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Anti-KIFAP3 Antibody (VS3-FY2018) in WB.
The KIFAP3 expression in A549, HL-60, C6 lysates were detected using the anti-KIFAP3 by western blot assay.
Figure 2 Anti-KIFAP3 Antibody (VS3-FY2018) in IHC.
Immunohistochemistry staining of paraffin-embedded human lung cancer using the anti-KIFAP3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Specifications
- Immunogen
- Synthetic peptide of human KIFAP3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat KIFAP3
- Species Reactivity
- Human, Rat
- Clone
- R03-6C6
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 91 kDa, while the observed molecular weight stands at 91 kDa.
- Related Disease
- Human lung cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Kinesin Associated Protein 3; Kinesin-Associated Protein 3; Smg GDS-Associated Protein; KAP-3; SMAP; KAP3; Small G Protein GDP Dissociation Stimulator
- Gene ID
- 22920
- UniProt ID
- Q92845
- Post Translation Modifications
- Phosphorylated on tyrosine residues by SRC in vitro; this reduces the binding affinity of the protein for RAP1GDS1. (Q92845-KIFA3_HUMAN)
Ubiquitination at Lys59, Lys469, Lys477, and Lys485 (NX_Q92845 [NX_Q92845-1])
Modification sites at PhosphoSitePlus (Q92845)
Glycosylation from GlyGen (Q92845) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_024309954.1; XP_047305487.1; XP_047305488.1; XP_047305493.1; XP_054191187.1
- Function
- Involved in tethering the chromosomes to the spindle pole and in chromosome movement. Binds to the tail domain of the KIF3A/KIF3B heterodimer to form a heterotrimeric KIF3 complex and may regulate the membrane binding of this complex (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "KIFAP3"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1942 | Hi-Affi™ Recombinant Rabbit Anti-KIFAP3 Monoclonal Antibody (DS1942AB) | WB, IHC-P, ICC | IgG |
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