Rabbit Anti-KIFAP3 Recombinant Antibody (clone R03-6C6) (CAT#: VS3-FY2018)
Clone R03-6C6 is a rabbit recombinant monoclonal antibody that firmly attaches to human KIFAP3. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IHC-P. The KIFAP3 antibody has been teted positive by western blot analysis of KIFAP3 in A549, HL-60, C6 lysates and immunohistochemistry analysis of paraffin-embedded human lung cancer.
Specific Inquiry
WB
Figure 1 Anti-KIFAP3 Antibody (VS3-FY2018) in WB.
The KIFAP3 expression in A549, HL-60, C6 lysates were detected using the anti-KIFAP3 by western blot assay.
IHC
Figure 2 Anti-KIFAP3 Antibody (VS3-FY2018) in IHC.
Immunohistochemistry staining of paraffin-embedded human lung cancer using the anti-KIFAP3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Specifications
- Immunogen
- Synthetic peptide of human KIFAP3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat KIFAP3
- Species Reactivity
- Human, Rat
- Clone
- R03-6C6
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 91 kDa, while the observed molecular weight stands at 91 kDa.
- Related Disease
- Human lung cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Kinesin Associated Protein 3; Kinesin-Associated Protein 3; Smg GDS-Associated Protein; KAP-3; SMAP; KAP3; Small G Protein GDP Dissociation Stimulator
- Gene ID
- 22920
- UniProt ID
- Q92845
- Post Translation Modifications
- Phosphorylated on tyrosine residues by SRC in vitro; this reduces the binding affinity of the protein for RAP1GDS1. (Q92845-KIFA3_HUMAN)
Ubiquitination at Lys59, Lys469, Lys477, and Lys485 (NX_Q92845 [NX_Q92845-1])
Modification sites at PhosphoSitePlus (Q92845)
Glycosylation from GlyGen (Q92845) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_024309954.1; XP_047305487.1; XP_047305488.1; XP_047305493.1; XP_054191187.1
- Function
- Involved in tethering the chromosomes to the spindle pole and in chromosome movement. Binds to the tail domain of the KIF3A/KIF3B heterodimer to form a heterotrimeric KIF3 complex and may regulate the membrane binding of this complex (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Please select product type
Rabbit Monoclonal Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1942 | Hi-Affi™ Recombinant Rabbit Anti-KIFAP3 Monoclonal Antibody (DS1942AB) | WB, IHC-P, ICC | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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