Mouse Anti-KRT8 Recombinant Antibody (clone ABT-CK8)
CAT#: VS3-CJ425
This product is a mouse antibody that recognizes human KRT8.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/131/if_selected.jpg
Subcellular Location
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach, upper shows strong cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/ihc_selected.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells
Staining:
High
Intensity: Strong
Enterocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining:
High
Intensity: Strong
Goblet cells
Staining:
High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Spermatogonia cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_5_6.jpg
Normal Tissue
Figure 8 Prostate
Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_3_5.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (A549, BEWO, CACO-2, CAPAN-2, MCF7, SK-BR-3, T-47d)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170421-KRT8
❮
❯
Specifications
- Immunogen
- Synthesized peptide derived from human Cytokeratin 8
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human KRT8
- Species Reactivity
- Human
- Clone
- ABT-CK8
- Applications
- WB, IHC-P
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.5% BSA, PBS.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:500-1:2000), Immunohistochemistry-Parraffin (1:100-1:500).
Target
- Alternative Names
- K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
- Gene ID
- 3856
- UniProt ID
- P05787
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cytoplasm, Intermediate filament, Keratin, Nucleus
- Post Translation Modifications
- Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
- Protein Refseq
- NP_001243222.1; NP_002264.1
- Function
- Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "KRT8"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1179z | Mouse Anti-KRT8 Recombinant Antibody (clone 20C2) | FC, ICC, IF, IHC | Mouse IgG1, κ |
| ZG-0327U | Rabbit Anti-KRT8 Recombinant Antibody (clone R15-K) | IHC-P | Rabbit IgG |
| VS3-QX665 | Mouse Anti-KRT8 Recombinant Antibody (clone 2F4-H9-D2) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-XY3413 | Rabbit Anti-KRT8 Recombinant Antibody (clone R01-9E6) | WB, IHC-P, ICC, IF, IP, FC | Rabbit IgG |
| VS7-0425-WR272 | Mouse Anti-KRT8 Monoclonal Antibody (VS7-0425-WR272) | WB, IHC-P, IF, ICC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1993 | Hi-Affi™ Recombinant Rabbit Anti-KRT8 Monoclonal Antibody (DS1993AB) | IHC-P, IHC-Fr | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0199-WJ-S(P) | Mouse Anti-KRT8 Recombinant Antibody (clone 8G8); scFv Fragment | ELISA, IHC | Mouse scFv |
| FAMAB-0344JF-S(P) | Human Anti-KRT8 Recombinant Antibody (clone COU-1); scFv Fragment | IHC, ELISA, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0199-WJ-F(E) | Mouse Anti-KRT8 Recombinant Antibody (clone 8G8); Fab Fragment | ELISA, IHC | Mouse Fab |
| FAMAB-0344JF-F(E) | Human Anti-KRT8 Recombinant Antibody (clone COU-1); Fab Fragment | IHC, ELISA, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0344JF | Human Anti-KRT8 Recombinant Antibody (clone COU-1) | WB, ELISA, IHC, FC | Human IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1415 | AbPlus™ Anti-KRT8 Magnetic Beads (VS-0724-YC1415) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3897 | Anti-KRT8 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3898 | Anti-Mouse KRT8 Immunohistochemistry Kit | IHC |
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