Rabbit Anti-LDHD Antibody (VS-0924-YC68) - Cancer Stem Cell Marker (CAT#: VS-0924-YC68)
Introducing the anti-LDHD antibody (VS-0924-YC68). This antibody specifically targets the human LDHD protein, which serves as a marker for cancer stem cells (CSCs). It is suitable for detecting LDHD in CSC tissues or cells using Immunohistochemistry at dilutions of 1/200-1/500, and Western blot at concentrations of 0.1-0.5 µg/mL.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/66148/1354_G8_4_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/66148/1354_G8_4_selected.jpg
Figure 2 IHC staining of human kidney
(High expression. RNA expression: 36.1 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48639/111488_A_9_5_val_selected.jpg
(High expression. RNA expression: 36.1 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48639/111488_A_9_5_val_selected.jpg
Figure 3 IHC staining of human testis
(Low expression. RNA expression: 1.7 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48639/111488_A_5_6_val_selected.jpg
(Low expression. RNA expression: 1.7 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48639/111488_A_5_6_val_selected.jpg
Figure 4 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41766/91779_B_9_5.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41766/91779_B_9_5.jpg
Figure 5 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41766/91779_A_7_3.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41766/91779_A_7_3.jpg
Figure 6 Liver
(Cholangiocytes
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41766/91779_A_7_4.jpg
(Cholangiocytes
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41766/91779_A_7_4.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41766/91779_A_9_8.jpg
(Germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41766/91779_A_9_8.jpg
Figure 8 RNA cell line category: Cell line enhanced (Hep G2, HMC-1, T-47d, U-266/70)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166816-LDHD
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166816-LDHD
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human LDHD
- Species Reactivity
- Human
- Applications
- IHC, WB
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity purified using the PrEST antigen as affinity ligand
- Concentration
- 0.2 mg/mL
- Buffer
- PBS, pH 7.2, with 40% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry (IHC): 1/200-1/500
Western Blot (WB): 0.1-0.5 µg/mL
Target
- Alternative Names
- DLD; DLACD
- Gene ID
- 197257
- UniProt ID
- Q86WU2
- Long Name
- Lactate dehydrogenase D
- Cellular Localization
- #Mitochondrion
- Function
- #Oxidoreductase
Recommended Products
Isotype Control
Secondary Antibody
Downloads
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Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2032 | Hi-Affi™ Recombinant Rabbit Anti-LDHD Monoclonal Antibody (DS2032AB) | WB | IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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