Mouse Anti-MAP2K2 Monoclonal Antibody (clone 7F5) (CAT#: VS7-0425-WR332)
This product is a mouse IgG1 antibody that binds to human MAP2K2. The antibody 7F5 can be used for WB, ICC, and FC assays. It cross reacts with mouse and rat.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51993/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51993/if_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3835/640_C4_2_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3835/640_C4_2_selected.jpg
Figure 3 IHC staining of human small intestine
(Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51993/ihc_selected.jpg
(Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51993/ihc_selected.jpg
Figure 4 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3835/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3835/ihc_selected.jpg
Figure 5 Skin
(Epidermal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3835/11077_B_4_8.jpg
(Epidermal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3835/11077_B_4_8.jpg
Figure 6 Lymph node
(Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3835/11077_A_7_8.jpg
(Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3835/11077_A_7_8.jpg
Figure 7 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3835/11077_A_7_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3835/11077_A_7_4.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000126934-MAP2K2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000126934-MAP2K2
Specifications
- Immunogen
- Recombinant human MAP2K2 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human MAP2K2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 7F5
- Molecule Class
- Map Kinase Kinase
- Applications
- WB, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Cardiofaciocutaneous syndrome 4 (CFC4)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- CFC4; MEK2; MKK2; MAPKK2; PRKMK2
- Gene ID
- 5605
- UniProt ID
- P36507
- Long Name
- Mitogen-Activated Protein Kinase Kinase 2
- Cellular Localization
- Cytoplasm; Membrane
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_109587.1
- Function
- Kinase; Serine/threonine-protein kinase; Transferase; Tyrosine-protein kinase
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
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| MOR-2132 | Hi-Affi™ Recombinant Rabbit Anti-MAP2K2 Monoclonal Antibody (DS2132AB) | WB, FC, IP, IHC | IgG |
| MOR-4643 | Hi-Affi™ Recombinant Rabbit Anti-MAP2K2 Monoclonal Antibody (TH156DS) | WB, IF, ICC, FC | IgG |
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