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Rabbit Anti-MAT1A Recombinant Antibody (clone R09-9C8) (CAT#: VS3-FY1763)

Clone R09-9C8 is a rabbit recombinant monoclonal antibody that confidently identify human MAT1A protein, a calculated weight of 44 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R09-9C8 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunoprecipitation (dilution range 1:20).


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Normal Tissue
Figure 1 IHC staining of human liver
Figure 1 IHC staining of human liver
(Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48627/ihc_selected.jpg
Figure 2 Cerebral cortex
Figure 2 Cerebral cortex
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48627/110760_B_7_5.jpg
Figure 3 Colon
Figure 3 Colon
(Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48627/110760_A_9_3.jpg
Figure 4 Liver
Figure 4 Liver
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48627/110760_A_9_4.jpg
Figure 5 Lymph node
Figure 5 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48627/110760_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enriched (Hep G2)
Figure 6 RNA cell line category: Cell line enriched (Hep G2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000151224-MAT1A

Specifications

  • Immunogen
  • Synthetic peptide of human MAT1A
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat MAT1A
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R09-9C8
  • Applications
  • Western Blot, Immunoprecipitation
  • MW
  • The calculated weight of the target protein is approximately 44 kDa, while the observed molecular weight stands at 44 kDa.
  • Related Disease
  • Methionine Adenosyltransferase I/Iii Deficiency and Hypermethioninemia

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • Methionine Adenosyltransferase 1A; Methionine Adenosyltransferase I, Alpha; Methionine Adenosyltransferase I/III; Methionine Adenosyltransferase 1; AdoMet Synthase 1; EC 2.5.1.6; MAT-I/III; MAT 1; MATA1; S-Adenosylmethionine Synthetase Isoform Type-1
  • Sequence Similarities
  • Belongs to the AdoMet synthase family.
  • Cellular Localization
  • Cytosol
  • Post Translation Modifications
  • S-nitrosylation of Cys-120 inactivates the enzyme.
    An intrachain disulfide bond can be formed. The protein structure shows that the relevant Cys residues are in a position that would permit formation of a disulfide bond.
  • Function
  • Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.

Recommended Products

Isotype Control

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2173 Hi-Affi™ Recombinant Rabbit Anti-MAT1A Monoclonal Antibody (DS2173AB) WB, IP, FC IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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