Rabbit Anti-MAT1A Recombinant Antibody (clone R09-9C8) (CAT#: VS3-FY1763)
Clone R09-9C8 is a rabbit recombinant monoclonal antibody that confidently identify human MAT1A protein, a calculated weight of 44 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R09-9C8 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunoprecipitation (dilution range 1:20).
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Normal Tissue
RNA Expression
Figure 1 IHC staining of human liver
(Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48627/ihc_selected.jpg
(Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48627/ihc_selected.jpg
Figure 2 Cerebral cortex
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48627/110760_B_7_5.jpg
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48627/110760_B_7_5.jpg
Figure 3 Colon
(Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48627/110760_A_9_3.jpg
(Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48627/110760_A_9_3.jpg
Figure 4 Liver
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48627/110760_A_9_4.jpg
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48627/110760_A_9_4.jpg
Figure 5 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48627/110760_A_8_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48627/110760_A_8_8.jpg
Figure 6 RNA cell line category: Cell line enriched (Hep G2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000151224-MAT1A
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000151224-MAT1A
Specifications
- Immunogen
- Synthetic peptide of human MAT1A
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat MAT1A
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R09-9C8
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 44 kDa, while the observed molecular weight stands at 44 kDa.
- Related Disease
- Methionine Adenosyltransferase I/Iii Deficiency and Hypermethioninemia
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Methionine Adenosyltransferase 1A; Methionine Adenosyltransferase I, Alpha; Methionine Adenosyltransferase I/III; Methionine Adenosyltransferase 1; AdoMet Synthase 1; EC 2.5.1.6; MAT-I/III; MAT 1; MATA1; S-Adenosylmethionine Synthetase Isoform Type-1
- Gene ID
- 4143
- UniProt ID
- Q00266
- Sequence Similarities
- Belongs to the AdoMet synthase family.
- Cellular Localization
- Cytosol
- Post Translation Modifications
- S-nitrosylation of Cys-120 inactivates the enzyme.
An intrachain disulfide bond can be formed. The protein structure shows that the relevant Cys residues are in a position that would permit formation of a disulfide bond.
- Protein Refseq
- NP_000420.1
- Function
- Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2173 | Hi-Affi™ Recombinant Rabbit Anti-MAT1A Monoclonal Antibody (DS2173AB) | WB, IP, FC | IgG |
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