Rabbit Anti-MELK Recombinant Antibody (clone R06-4D6)
CAT#: VS3-FY2034
The R06-4D6 recombinant monoclonal antibody was developed to identify human MELK. It cross-reacts with rat. This MELK antibody was used in western blot analysis of MELK in U2OS, C6 lysates. Western blot demonstrated that R06-4D6 recognized a target protein with a molecular weight of 75 kDa.
Gene Expression
Tested Data
Normal Tissue
Figure 1 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17214/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17214/41246_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17214/41246_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17214/41246_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17214/41246_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17214/41246_A_4_6.jpg
Normal Tissue
Figure 7 Placenta
Decidual cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17214/41246_A_1_7.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17214/41246_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165304-MELK
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WB
Figure 1 Anti-MELK Antibody (VS3-FY2034) in WB.
The MELK expression in U2OS, C6 lysates were detected using the anti-MELK by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human MELK
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat MELK
- Species Reactivity
- Human, Rat
- Clone
- R06-4D6
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 75 kDa, while the observed molecular weight stands at 75 kDa.
- Related Disease
- Uterine Corpus Endometrial Carcinom
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Maternal Embryonic Leucine Zipper Kinase; Tyrosine-Protein Kinase MELK; Protein Kinase PK38; Protein Kinase Eg3; PEg3 Kinase; EC 2.7.11.1
- Gene ID
- 9833
- UniProt ID
- Q14680
- Sequence Similarities
- Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Autophosphorylated: autophosphorylation of the T-loop at Thr-167 and Ser-171 is required for activation.
Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8 (Probable).
(Q14680-MELK_HUMAN)
Ubiquitination at Lys44, Lys395, Lys406, Lys434, Lys536, Lys597, and Lys650 (NX_Q14680 [NX_Q14680-1])
Modification sites at PhosphoSitePlus (Q14680)
Glycosylation from GlyGen (Q14680) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011516378.1; XP_011516379.1; XP_011516382.1; XP_011516383.1; XP_011516384.1
- Function
- Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis.
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# VS3-FY2034, RRID: AB_3714405)
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MELK"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2210 | Hi-Affi™ Recombinant Rabbit Anti-MELK Monoclonal Antibody (DS2210AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1037 | PE-A*24:02/Human MELK (EYCPGGNLF) MHC Tetramer | FCM, IHC | |
| MHC-CN1038 | APC-A*24:02/Human MELK (EYCPGGNLF) MHC Tetramer | FCM, IHC | |
| MHC-CN1039 | FITC-A*24:02/Human MELK (EYCPGGNLF) MHC Tetramer | FCM, IHC | |
| MHC-CN1040 | A*24:02/Human MELK (EYCPGGNLF) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-XY1060 | Mouse Anti-MELK Recombinant Antibody (clone 2G2) | ELISA, IHC, FC, ICC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1333 | Anti-MELK Immunohistochemistry Kit | IHC | |
| VS-0525-XY4349 | Anti-Human MELK Immunohistochemistry Kit | IHC |
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