Human Anti-MERS-CoV Recombinant Antibody (clone C8) (CAT#: FAMAB-0620WJ)
This product is a human monoclonal antibody that is specific for MERS-CoV. This antibody can be used in a variety of applications, such as ELISA, WB, Neut, IHC, FC.
Specific Inquiry
ELISA
Figure 1 Clones were selected from the second polyclonal pool for monoclonal ELISA assay against crude rMERS-NP.
Histogram showing the absorbance of monoclonal scFv antibody phage binding against crude rMERS-NP after normalizing with background values. H5 and H11 are the positive controls, labelled as P (OD405nm readouts 3.54 and 3.90, respectively) while H6 and H12 are the negative controls, labelled as N (OD405nm readouts 0).
Lim, C. C., Woo, P. C., & Lim, T. S. (2019). Development of a phage display panning strategy utilizing crude antigens: isolation of MERS-CoV nucleoprotein human antibodies. Scientific reports, 9(1), 1-15.
ELISA
Figure 2 The antibody fragments were subjected to antigen binding ELISA assay against a serial dilution of rMERS-NP(His6) at various concentrations.
The absorbance of each set of samples after normalizing with background values are presented as mean ± s.d. (n = 3) in the line graphs.
Lim, C. C., Woo, P. C., & Lim, T. S. (2019). Development of a phage display panning strategy utilizing crude antigens: isolation of MERS-CoV nucleoprotein human antibodies. Scientific reports, 9(1), 1-15.
ELISA
Figure 3 The absorbance of the control set during the ELISA assay.
Lim, C. C., Woo, P. C., & Lim, T. S. (2019). Development of a phage display panning strategy utilizing crude antigens: isolation of MERS-CoV nucleoprotein human antibodies. Scientific reports, 9(1), 1-15.
ELISA
Figure 4 Cross-reactivity ELISA assay of antibody fragments against other His6-tagged protein was also performed.
The control antigen used is 10 μg of rGFP(His6). The absorbance for all sample sets were collected in triplicates and presented as mean ± s.d. (n = 3) in the bar graph.
Lim, C. C., Woo, P. C., & Lim, T. S. (2019). Development of a phage display panning strategy utilizing crude antigens: isolation of MERS-CoV nucleoprotein human antibodies. Scientific reports, 9(1), 1-15.
Specifications
- Host Species
- Human
- Derivation
- Phage Display Library
- Type
- Human IgG
- Specificity
- MERS-CoV
- Species Reactivity
- MERS
- Clone
- C8
- Applications
- ELISA, WB, Neut
- Related Disease
- MERS infection
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Middle East respiratory syndrome-related coronavirus; MERS-CoV
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
ScFv Antibody Products
Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4106 | Hi-Affi™ Recombinant Rabbit Anti-MERS-CoV Monoclonal Antibody (SI177DS) | ELISA, IHC-P, ICC/IF, IF, FC | IgG |
| MOR-4453 | Hi-Affi™ Recombinant Rabbit Anti-MERS-CoV Monoclonal Antibody (SI546DS) | MN | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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