Human Anti-MERS-CoV Recombinant Antibody (clone C8) (CAT#: FAMAB-0620WJ)
This product is a human monoclonal antibody that is specific for MERS-CoV. This antibody can be used in a variety of applications, such as ELISA, WB, Neut, IHC, FC.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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ELISA
Figure 1 Clones were selected from the second polyclonal pool for monoclonal ELISA assay against crude rMERS-NP.
Histogram showing the absorbance of monoclonal scFv antibody phage binding against crude rMERS-NP after normalizing with background values. H5 and H11 are the positive controls, labelled as P (OD405nm readouts 3.54 and 3.90, respectively) while H6 and H12 are the negative controls, labelled as N (OD405nm readouts 0).
Lim, C. C., Woo, P. C., & Lim, T. S. (2019). Development of a phage display panning strategy utilizing crude antigens: isolation of MERS-CoV nucleoprotein human antibodies. Scientific reports, 9(1), 1-15.
ELISA
Figure 2 The antibody fragments were subjected to antigen binding ELISA assay against a serial dilution of rMERS-NP(His6) at various concentrations.
The absorbance of each set of samples after normalizing with background values are presented as mean ± s.d. (n = 3) in the line graphs.
Lim, C. C., Woo, P. C., & Lim, T. S. (2019). Development of a phage display panning strategy utilizing crude antigens: isolation of MERS-CoV nucleoprotein human antibodies. Scientific reports, 9(1), 1-15.
ELISA
Figure 3 The absorbance of the control set during the ELISA assay.
Lim, C. C., Woo, P. C., & Lim, T. S. (2019). Development of a phage display panning strategy utilizing crude antigens: isolation of MERS-CoV nucleoprotein human antibodies. Scientific reports, 9(1), 1-15.
ELISA
Figure 4 Cross-reactivity ELISA assay of antibody fragments against other His6-tagged protein was also performed.
The control antigen used is 10 μg of rGFP(His6). The absorbance for all sample sets were collected in triplicates and presented as mean ± s.d. (n = 3) in the bar graph.
Lim, C. C., Woo, P. C., & Lim, T. S. (2019). Development of a phage display panning strategy utilizing crude antigens: isolation of MERS-CoV nucleoprotein human antibodies. Scientific reports, 9(1), 1-15.
Specifications
- Host Species
- Human
- Derivation
- Phage Display Library
- Type
- Human IgG
- Specificity
- MERS-CoV
- Species Reactivity
- MERS
- Clone
- C8
- Applications
- ELISA, WB, Neut
- Related Disease
- MERS infection
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Middle East respiratory syndrome-related coronavirus; MERS-CoV
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "MERS-CoV"
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
ScFv Antibody Products
Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4106 | Hi-Affi™ Recombinant Rabbit Anti-MERS-CoV Monoclonal Antibody (SI177DS) | ELISA, IHC-P, ICC/IF, IF, FC | IgG |
| MOR-4453 | Hi-Affi™ Recombinant Rabbit Anti-MERS-CoV Monoclonal Antibody (SI546DS) | MN | IgG |
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For Research Use Only. Not For Clinical Use.
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