Anti-MSH2 (clone W24V219) Recombinant Antibody Coupled Liposome (VS-1024-FY216) (CAT#: VS-1024-FY216)

• Potential Clinical Applications
• Ovarian cancer

• Clone
• W24V219

• Antibody Type
• IgG1, kappa

• Antibody Host
• Mouse

• Antibody Reactivity
• Human

• Antibody Description
• This antibody is a recombinant mouse monoclonal that was specifically engineered to target MSH2, a crucial protein involved in DNA mismatch repair. By binding to MSH2, this antibody can enhance the understanding of genetic stability and its role in various cancers. Its specificity offers potential applications in both diagnostic and therapeutic settings, facilitating research into inherited diseases linked to MSH2 mutations.

• Storage
• See in the COA

• Storage Shelf Time
• See in the COA

• Target
• MSH2

• Introduction
• This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. Coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.

• Alternative Names
• MutS Homolog 2; HMSH2; MutS (E. Coli) Homolog 2 (Colon Cancer, Nonpolyposis Type 1); MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli); MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1; DNA Mismatch Repair Protein Msh2; MutS Protein Homolog 2

• Full Name
• MutS Homolog 2

• Gene ID
• 4436

• UniProt ID
• P43246

• Sequence Similarities
• Belongs to the DNA mismatch repair MutS family.

• Cellular Localization
• Chromosome, Nucleus

• Post Translation Modifications
• Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.

• Protein Refseq
• NP_000242.1; NP_001245210.1

• Function
• Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containing DNA strand (PubMed:26300262).
  ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

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