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  • Rabbit Anti-MT-ND1 Recombinant Antibody (clone R04-9H6)

Rabbit Anti-MT-ND1 Recombinant Antibody (clone R04-9H6) (CAT#: VS3-FY1778)

Clone R04-9H6 is a rabbit IgG recombinant antibody that is capable of recognizing human MT-ND1 protein, a calculated weight of 36 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R04-9H6 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000).


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Normal Tissue
Figure 1 IHC staining of human skeletal muscle
Figure 1 IHC staining of human skeletal muscle
(Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/66847/170387_B_2_6_selected.jpg
Figure 2 IHC staining of human heart muscle
Figure 2 IHC staining of human heart muscle
(Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33779/66375_B_4_6_selected.jpg
Figure 3 Cerebral cortex
Figure 3 Cerebral cortex
(Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33779/66375_B_7_5.jpg
Figure 4 Colon
Figure 4 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33779/66375_A_8_3.jpg
Figure 5 Kidney
Figure 5 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33779/66375_A_9_5.jpg
Figure 6 Lymph node
Figure 6 Lymph node
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33779/66375_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HeLa)
Figure 7 RNA cell line category: Cell line enhanced (HeLa)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000198888-mt-Nd1

Specifications

  • Immunogen
  • Synthetic peptide of human MT-ND1
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat MT-ND1
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R04-9H6
  • Applications
  • Western Blot
  • MW
  • The calculated weight of the target protein is approximately 36 kDa, while the observed molecular weight stands at 36 kDa.
  • Related Disease
  • Mitochondrial Myopathy, Encephalopathy, Lactic Acidosis, and Stroke-Like Episodes and Leber Hereditary Optic Neuropathy

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000

Target

  • Alternative Names
  • Mitochondrially Encoded NADH:Ubiquinone Oxidoreductase Core Subunit 1; NADH Dehydrogenase Subunit 1; EC 1.6.5.3; MTND1; ND1; Mitochondrially Encoded NADH Dehydrogenase 1
  • Sequence Similarities
  • Belongs to the complex I subunit 1 family.
  • Cellular Localization
  • Mitochondrion inner membrane
  • Function
  • Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor.

Recommended Products

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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