Rabbit Anti-MT-ND1 Recombinant Antibody (clone R04-9H6)
CAT#: VS3-FY1778
Clone R04-9H6 is a rabbit IgG recombinant antibody that is capable of recognizing human MT-ND1 protein, a calculated weight of 36 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R04-9H6 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000).
Gene Expression
Normal Tissue
Figure 1 IHC staining of human skeletal muscle
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66847/170387_B_2_6_selected.jpg
Normal Tissue
Figure 2 IHC staining of human heart muscle
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/33779/66375_B_4_6_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/33779/66375_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/33779/66375_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/33779/66375_A_9_5.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/33779/66375_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HeLa)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000198888-mt-Nd1
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Specifications
- Immunogen
- Synthetic peptide of human MT-ND1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat MT-ND1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-9H6
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 36 kDa, while the observed molecular weight stands at 36 kDa.
- Related Disease
- Mitochondrial Myopathy, Encephalopathy, Lactic Acidosis, and Stroke-Like Episodes and Leber Hereditary Optic Neuropathy
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Mitochondrially Encoded NADH:Ubiquinone Oxidoreductase Core Subunit 1; NADH Dehydrogenase Subunit 1; EC 1.6.5.3; MTND1; ND1; Mitochondrially Encoded NADH Dehydrogenase 1
- Gene ID
- 4535
- UniProt ID
- P03886
- Sequence Similarities
- Belongs to the complex I subunit 1 family.
- Cellular Localization
- Mitochondrion inner membrane
- Protein Refseq
- YP_003024026.1
- Function
- Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "mt-Nd1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1046 | PE-H2-M3/Rat mt-Nd1 (MYFINILTL) MHC Tetramer | FCM | |
| MHC-LC1047 | APC-H2-M3/Rat mt-Nd1 (MYFINILTL) MHC Tetramer | FCM | |
| MHC-LC1048 | BV421-H2-M3/Rat mt-Nd1 (MYFINILTL) MHC Tetramer | FCM |
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