Anti-MYH9 Antibody Prodrug, Protease Activated (CLL412)
CAT#: VS-1025-YC168
The Anti-MYH9 Antibody Prodrug, Protease Activated (CLL412) is an antibody prodrug comprised of a recombinant human antibody targeting human MYH9, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to MYH9 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to MYH9 effectively. MYH9 (Myosin Heavy Chain 9) is a cytoskeletal motor protein whose overexpression promotes tumor cell invasion and metastasis by driving cell contractility and motility. It is associated with poor prognosis in various solid tumors.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & actin filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1644/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies, plasma membrane & actin filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/1685_C7_1_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies, plasma membrane & actin filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1644/62_G1_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_A_8_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_A_6_6.jpg
Normal Tissue
Figure 8 Endometrium
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_B_6_3.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100345-MYH9
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Specifications
- Host Animal
- Human
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human MYH9
- Target
- MYH9
- Species Reactivity
- Human
- Isotype
- IgG1
- Clone
- CLL412
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- This gene encodes a conventional non-muscle myosin; this protein should not be confused with the unconventional myosin-9a or 9b (MYO9A or MYO9B). The encoded protein is a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain which is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in this gene have been associated with non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness.
- Alternative Names
- Myosin Heavy Chain 9; Cellular Myosin Heavy Chain, Type A; NMMHC-IIA; Myosin, Heavy Polypeptide 9, Non-Muscle; Non-Muscle Myosin Heavy Polypeptide 9; Myosin Heavy Chain, Non-Muscle Iia
- Gene ID
- 4627
- UniProt ID
- P35579
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Datasheet
MSDS
COA
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See other products for "Clone CLL412"
- CAT
- Product Name
See other products for "Myh9"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-999 | Recombinant Human Anti-Rat Myh9 Antibody,clone SF9 | ELISA, ICC, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0795CT | Recombinant Mouse anti-Human MYH9 Monoclonal antibody (EML521) | FC, ICC, IF, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2341 | Hi-Affi™ Recombinant Rabbit Anti-MYH9 Monoclonal Antibody (DS2341AB) | WB, IHC, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC3449 | PE-A*02:01/Human MYH9 (YLYVDKNFI) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC3450 | PE-A*02:06/Human MYH9 (YLYVDKNFI) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC3451 | PE-A*68:02/Human MYH9 (YLYVDKNFI) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC3452 | PE-A*02:03/Human MYH9 (YLYVDKNFI) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC3453 | PE-A*02:02/Human MYH9 (YLYVDKNFI) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1997-FY | Human Anti-MYH9 Recombinant Antibody (clone CLL412) | IP | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1997-FY-F(E) | Human Anti-MYH9 Recombinant Antibody (clone CLL412); Fab Fragment | IP | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1997-FY-S(P) | Human Anti-MYH9 Recombinant Antibody (clone CLL412); scFv Fragment | IP | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1998-FY | Human Anti-MYH9 Recombinant Antibody (clone CLL068) | IP, IHC, IF | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1998-FY-F(E) | Human Anti-MYH9 Recombinant Antibody (clone CLL068); Fab Fragment | IP, IHC, IF | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1998-FY-S(P) | Human Anti-MYH9 Recombinant Antibody (clone CLL068); scFv Fragment | IP, IHC, IF | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1999-FY | Human Anti-MYH9 Recombinant Antibody (clone CLL258) | IP, IHC | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1999-FY-F(E) | Human Anti-MYH9 Recombinant Antibody (clone CLL258); Fab Fragment | IP, IHC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1999-FY-S(P) | Human Anti-MYH9 Recombinant Antibody (clone CLL258); scFv Fragment | IP, IHC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1415 | Anti-MYH9 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4636 | Anti-Mouse MYH9 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4635 | Anti-Human MYH9 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4637 | Anti-Canine MYH9 Immunohistochemistry Kit | IHC |
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