Recombinant Mouse anti-Human MYH9 Monoclonal antibody (EML521)
CAT#: MOB-0795CT
Recombinant Mouse monoclonal antibody to Human MYH9, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & actin filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1644/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies, plasma membrane & actin filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/1685_C7_1_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies, plasma membrane & actin filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1644/62_G1_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_A_8_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_A_6_6.jpg
Normal Tissue
Figure 8 Endometrium
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_B_6_3.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100345-MYH9
❮
❯
Specifications
- Immunogen
- Recombinant fragment: RLKQLKRQLE EAEEEAQRAN ASRRKLQREL EDATETADAM NREVSSLKNK LRRGDLPFVV PRRMARKGAG DGSDEEVDGK ADGAEAKPAE , corresponding to amino acids 131-221 of Human non-muscle Myosin IIA
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- EML521
- Applications
- Used for immunoassay techniques such as: Flow Cytometry; Immunocytochemistry/Immunofluorescence; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein G purified
- Format
- Liquid
- Concentration
- Batch dependent within range:0.5 -1.0 mg/ml
- Buffer
- Preservative: None PBS, pH 7.2
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- MYH9; myosin, heavy chain 9, non-muscle; MHA; FTNS; EPSTS; BDPLT6; DFNA17; NMMHCA; NMHC-II-A; NMMHC-IIA; myosin-9; non-muscle myosin heavy chain A; non-muscle myosin heavy chain IIa; nonmuscle myosin heavy chain II-A; cellular myosin heavy chain, type A; non-muscle myosin heavy polypeptide 9;
- Gene ID
- 4627
- UniProt ID
- P35579
- Protein Refseq
- NP_002464
- Function
- ADP binding; ATP binding; ATP binding; ATPase activity; actin binding; actin binding; actin filament binding; actin filament binding; actin-dependent ATPase activity; calmodulin binding; microfilament motor activity; motor activity; motor activity; nucleo
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Myh9"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-999 | Recombinant Human Anti-Rat Myh9 Antibody,clone SF9 | ELISA, ICC, WB | IgG |
| HPAB-1997-FY | Human Anti-MYH9 Recombinant Antibody (clone CLL412) | IP | Human IgG1 |
| HPAB-1998-FY | Human Anti-MYH9 Recombinant Antibody (clone CLL068) | IP, IHC, IF | Human IgG1 |
| HPAB-1999-FY | Human Anti-MYH9 Recombinant Antibody (clone CLL258) | IP, IHC | Human IgG1 |
| VS3-FY993 | Recombinant Rabbit Anti-MYH9 Antibody (clone R08-2C2) | WB, IHC-P, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2341 | Hi-Affi™ Recombinant Rabbit Anti-MYH9 Monoclonal Antibody (DS2341AB) | WB, IHC, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1997-FY-F(E) | Human Anti-MYH9 Recombinant Antibody (clone CLL412); Fab Fragment | IP | Human Fab |
| HPAB-1998-FY-F(E) | Human Anti-MYH9 Recombinant Antibody (clone CLL068); Fab Fragment | IP, IHC, IF | Human Fab |
| HPAB-1999-FY-F(E) | Human Anti-MYH9 Recombinant Antibody (clone CLL258); Fab Fragment | IP, IHC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1997-FY-S(P) | Human Anti-MYH9 Recombinant Antibody (clone CLL412); scFv Fragment | IP | Human scFv |
| HPAB-1998-FY-S(P) | Human Anti-MYH9 Recombinant Antibody (clone CLL068); scFv Fragment | IP, IHC, IF | Human scFv |
| HPAB-1999-FY-S(P) | Human Anti-MYH9 Recombinant Antibody (clone CLL258); scFv Fragment | IP, IHC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1415 | Anti-MYH9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4636 | Anti-Mouse MYH9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4635 | Anti-Human MYH9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4637 | Anti-Canine MYH9 Immunohistochemistry Kit | IHC |
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