Rabbit Anti-NCS1 Recombinant Antibody (clone R35-9B-6)
CAT#: VS3-FY2139
Clone R35-9B-6 is a rabbit recombinant monoclonal antibody that has been genetically engineered to specifically target human NCS1 protein, a calculated weight of 22 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R35-9B-6 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200), flow cytometry (dilution range 1:50).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19713/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18587/if_selected.jpg
Normal Tissue
Figure 3 High expression in cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18587/40690_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18587/40690_A_9_3.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107130-NCS1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat NCS1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R35-9B-6
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Flow Cytometry
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 22 kDa, while the observed molecular weight stands at 22kDa.
- Related Disease
- Schizophrenia and Wolfram Syndrome 2
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Flow cytometry: 1/50
Target
- Alternative Names
- Neuronal Calcium Sensor 1; Frequenin-Like Ubiquitous Protein; Frequenin-Like Protein; Frequenin Homolog; FREQ; FLUP; Frequenin (Drosophila) Homolog; Frequenin Homolog (Drosophila); NCS-1
- Gene ID
- 23413
- UniProt ID
- P62166
- Sequence Similarities
- Belongs to the recoverin family.
- Cellular Localization
- Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Synapse
- Post Translation Modifications
- Ubiquitination at Lys25 and Lys53 (NX_P62166 [NX_P62166-1])
Modification sites at PhosphoSitePlus (P62166)
- Protein Refseq
- NP_001122298.1; NP_055101.2
- Function
- Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin (By similarity).
Stimulates PI4KB kinase activity (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NCS1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2290z | Mouse Anti-NCS1 Recombinant Antibody (clone 22H11) | ELISA, WB | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-1033MZ | Chicken Anti-NCS1 Polyclonal IgY | ELISA, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2385 | Hi-Affi™ Recombinant Rabbit Anti-NCS1 Monoclonal Antibody (DS2385AB) | WB, IHC, IP, FC | IgG |
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