Rabbit Anti-NDEL1 Recombinant Antibody (clone R05-1D0) (CAT#: VS3-FY2043)
The R05-1D0 recombinant monoclonal antibody has been genetically engineered to specifically target human NDEL1. It cross-reacts with mouse. Applications Tested: The NDEL1 antibody has been tested by western blot analysis of NDEL1 in Hela, A549, HL-60, U251, U87-MG lysates.
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WB
Figure 1 Anti-NDEL1 Antibody (VS3-FY2043) in WB.
The NDEL1 expression in Hela, A549, HL-60, U251, U87-MG lysates were detected using the anti-NDEL1 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human NDEL1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse NDEL1
- Species Reactivity
- Human, Mouse
- Clone
- R05-1D0
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 38 kDa, while the observed molecular weight stands at 38 kDa.
- Related Disease
- Lissencephaly and Miller-Dieker Lissencephaly Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- NudE Neurodevelopment Protein 1 Like 1; Mitosin-Associated Protein 1; Protein Nudel; MITAP1; NUDEL; EOPA; NudE Nuclear Distribution Gene E Homolog (A. Nidulans)-Like 1; NudE Nuclear Distribution E Homolog (A. Nidulans)-Like 1
- Gene ID
- 81565
- UniProt ID
- Q9GZM8
- Sequence Similarities
- Belongs to the nudE family.
- Cellular Localization
- Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule
- Post Translation Modifications
- Phosphorylated in mitosis.
Can be phosphorylated by CDK1, CDK5 and MAPK1.
Phosphorylation by CDK5 promotes interaction with KATNA1 and YWHAE.
(Q9GZM8-NDEL1_HUMAN)
Palmitoylation at Cys-273 reduces affinity for dynein. (Q9GZM8-NDEL1_HUMAN)
Modification sites at PhosphoSitePlus (Q9GZM8)
Modification sites at neXtProt (NX_Q9GZM8)
Glycosylation from GlyGen (Q9GZM8) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_016880672.1; XP_016880673.1; XP_016880675.1; XP_016880677.1; XP_047292816.1
- Function
- May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibodies
IgG Antibodies
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2388 | Hi-Affi™ Recombinant Rabbit Anti-NDEL1 Monoclonal Antibody (DS2388AB) | WB, IHC | IgG |
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For Research Use Only. Not For Clinical Use.
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