Rabbit Anti-NDUFB1 Recombinant Antibody (clone R04-4H3)
CAT#: VS3-FY1948
The R04-4H3 recombinant monoclonal antibody could potentially be used to target human NDUFB1. It is suitable for use in a variety of assays, including WB, IHC-P. The binding specificity of the NDUFB1 antibody was determined using western blot analysis of NDUFB1 in A549, HL-60, U2OS lysates.
Tested Data
WB
Figure 1 Anti-NDUFB1 Antibody (VS3-FY1948) in WB.
The NDUFB1 expression in A549, HL-60, U2OS lysates were detected using the anti-NDUFB1 by western blot assay.
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Specifications
- Immunogen
- Recombinant human NDUFB1 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NDUFB1
- Species Reactivity
- Human
- Clone
- R04-4H3
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 7 kDa, while the observed molecular weight stands at 7 kDa.
- Related Disease
- Bacterial Gastritis and Mitochondrial Dna Depletion Syndrome 9
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- NADH:Ubiquinone Oxidoreductase Subunit B1; NADH Dehydrogenase (Ubiquinone) 1 Beta Subcomplex, 1, 7kDa; NADH-Ubiquinone Oxidoreductase MNLL Subunit; Complex I MNLL Subunit; Complex I-MNLL; CI-MNLL
- Gene ID
- 4707
- UniProt ID
- O75438
- Sequence Similarities
- Belongs to the complex I NDUFB1 subunit family.
- Cellular Localization
- Membrane, Mitochondrion, Mitochondrion inner membrane
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (O75438)
Modification sites at neXtProt (NX_O75438)
- Protein Refseq
- XP_047287377.1; NP_004536.3
- Function
- Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NDUFB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4739 | Anti-NDUFB1 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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