Rabbit Anti-NDUFS8 Recombinant Antibody (clone R09-2B2)
CAT#: VS3-FY1796
This R09-2B2 recombinant monoclonal antibody has the ability to specifically bind to human NDUFS8. It cross-reacts with mouse, rat. This NDUFS8 antibody has been rigorously validated and is widely used in western blot analysis of NDUFS8 in rat brain lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18524/if_selected.jpg
Normal Tissue
Figure 2 Heart muscle
High expression in heart muscle
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67429/169043_B_5_6_selected.jpg
Normal Tissue
Figure 3 Skeletal muscle
Myocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18524/41985_B_3_6.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18524/41985_A_9_5.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000110717-NDUFS8
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WB
Figure 1 Anti-NDUFS8 Antibody (VS3-FY1796) in WB.
The NDUFS8 expression in rat Brain lysates were detected using the anti-NDUFS8 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human NDUFS8
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat NDUFS8
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R09-2B2
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 24 kDa, while the observed molecular weight stands at 24 kDa.
- Related Disease
- Mitochondrial Complex Ii Deficiency, Nuclear Type 1 and Isolated Complex I Deficiency
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- NADH:Ubiquinone Oxidoreductase Core Subunit S8; NADH Dehydrogenase (Ubiquinone) Fe-S Protein 8, 23kDa (NADH-Coenzyme Q Reductase); NADH Dehydrogenase [Ubiquinone] Iron-Sulfur Protein 8, Mitochondrial; NADH-Ubiquinone Oxidoreductase 23 KDa Subunit; Complex I 23kDa Subunit; Complex I-23kD; NADH Dehydrogenase (Ubiquinone) Fe-S Protein 8 (23kD) (NADH-Coenzyme Q Reductase); TYKY Subunit
- Gene ID
- 4728
- UniProt ID
- O00217
- Sequence Similarities
- Belongs to the complex I 23 kDa subunit family.
- Cellular Localization
- Membrane, Mitochondrion, Mitochondrion inner membrane
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (O00217)
Modification sites at neXtProt (NX_O00217)
Glycosylation from GlyGen (O00217) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_002487.1
- Function
- Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NDUFS8"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3187z | Mouse Anti-NDUFS8 Recombinant Antibody (clone 23D5) | WB, IP, IF, ELISA | Mouse IgG1, κ |
| MOB-2402CT | Recombinant Mouse anti-Human NDUFS8 Monoclonal antibody (4) | WB | |
| VS13-YC779 | Rabbit Anti-NDUFS8 Recombinant Antibody (VS13-YC779) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2405 | Hi-Affi™ Recombinant Rabbit Anti-NDUFS8 Monoclonal Antibody (DS2405AB) | WB, IHC-P, ICC, IF, IP, FC | IgG |
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