Rabbit Anti-NEK2 Recombinant Antibody (clone R03-3D8)
CAT#: VS3-FY2045
The R03-3D8 recombinant monoclonal antibody has the ability to specifically bind to human NEK2. The binding specificity of the NEK2 antibody was determined using western blot analysis of NEK2 in Hela, A549, HL-60 lysates.
Tested Data
Gene Expression
WB
Figure 1 Anti-NEK2 Antibody (VS3-FY2045) in WB.
The NEK2 expression in Hela, A549, HL-60 lysates were detected using the anti-NEK2 by western blot assay.
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Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm & centrosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64967/1151_B3_4_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & centrosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17530/952_G4_3_selected.jpg
Normal Tissue
Figure 3 High expression in testis.
Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17530/164057_A_6_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17530/164057_A_8_8.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117650-NEK2
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Specifications
- Immunogen
- Synthetic peptide of human NEK2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NEK2
- Species Reactivity
- Human
- Clone
- R03-3D8
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 52 kDa, while the observed molecular weight stands at 52 kDa.
- Related Disease
- Retinitis Pigmentosa 67 and Retinitis Pigmentosa
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- NIMA Related Kinase 2; NIMA (Never In Mitosis Gene A)-Related Kinase 2; Protein Phosphatase 1, Regulatory Subunit 111; NimA-Related Protein Kinase 2; NimA-Like Protein Kinase 1; EC 2.7.11.1; HsPK 21; NEK2A
- Gene ID
- 4751
- UniProt ID
- P51955
- Sequence Similarities
- Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
- Cellular Localization
- Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore
- Post Translation Modifications
- Activated by autophosphorylation.
Protein phosphatase 1 represses autophosphorylation and activation of isoform 1 by dephosphorylation.
Phosphorylation by STK3/MST2 is necessary for its localization to the centrosome.
(P51955-NEK2_HUMAN)
Ubiquitination at Lys37, Lys152, Lys174, Lys359, and Lys362 (NX_P51955 [NX_P51955-1])
Modification sites at PhosphoSitePlus (P51955)
- Protein Refseq
- NP_001191111.1; NP_001191112.1; NP_002488.1
- Function
- Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGO1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NEK2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1331MZ | Recombinant Mouse Anti-Human NEK2 Antibody | IHC-P, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2415 | Hi-Affi™ Recombinant Rabbit Anti-NEK2 Monoclonal Antibody (DS2415AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS1-YJ139 | Sheep Anti-NEK2 Antibody (VS-0722-YJ139) | WB | Sheep IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1478 | Anti-NEK2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4786 | Anti-Human NEK2 Immunohistochemistry Kit | IHC |
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