Rabbit Anti-NEK2 Recombinant Antibody (clone R03-3D8) (CAT#: VS3-FY2045)

• Immunogen
• Synthetic peptide of human NEK2

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human NEK2

• Species Reactivity
• Human

• Clone
• R03-3D8

• Applications
• Western Blot

• MW
• The calculated weight of the target protein is approximately 52 kDa, while the observed molecular weight stands at 52 kDa.

• Related Disease
• Retinitis Pigmentosa 67 and Retinitis Pigmentosa

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000

• Alternative Names
• NIMA Related Kinase 2; NIMA (Never In Mitosis Gene A)-Related Kinase 2; Protein Phosphatase 1, Regulatory Subunit 111; NimA-Related Protein Kinase 2; NimA-Like Protein Kinase 1; EC 2.7.11.1; HsPK 21; NEK2A

• Gene ID
• 4751

• UniProt ID
• P51955

• Sequence Similarities
• Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.

• Cellular Localization
• Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore

• Post Translation Modifications
• Activated by autophosphorylation.
  Protein phosphatase 1 represses autophosphorylation and activation of isoform 1 by dephosphorylation.
  Phosphorylation by STK3/MST2 is necessary for its localization to the centrosome.
  (P51955-NEK2_HUMAN)
  Ubiquitination at Lys37, Lys152, Lys174, Lys359, and Lys362 (NX_P51955 [NX_P51955-1])
  Modification sites at PhosphoSitePlus (P51955)

• Protein Refseq
• NP_001191111.1; NP_001191112.1; NP_002488.1

• Function
• Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGO1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

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