Rabbit Anti-NFIB Recombinant Antibody (VS13-YC796)
CAT#: VS13-YC796
The Rabbit Anti-NFIB Recombinant Antibody is specific for human and rat NFIB. NFIB recognizes palindromic sequences in promoters and replication origins, individually activating transcription and replication. The anti-NFIB antibody can be used for Western blot (1/500-1/1000), Immunoprecipitation (1/20).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3956/28_H11_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human heart muscle
Immunohistochemical staining of human heart muscle shows strong nuclear positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3956/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3956/10623_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells Staining: Medium Intensity: Moderate Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Enterocytes Staining: Low Intensity: Moderate Quantity: <25% Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3956/10623_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3956/10623_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3956/10623_A_8_5.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (AN3-CA, HBEC3-KT, HEL, HUVEC TERT2, TIME)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147862-NFIB
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Specifications
- Immunogen
- Recombinant human NFIB.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat NFIB
- Species Reactivity
- Human, Rat
- Applications
- WB, IP
- Conjugate
- NONE
- Related Disease
- Macrocephaly, Acquired, With Impaired Intellectual Development and Adenoid Cystic Carcinoma
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4) containing 150 mM NaCl, 40% Glycerol and 0.05% BSA.
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunoprecipitation (IP): 1/20
Target
- Alternative Names
- Nuclear Factor I B; CCAAT-Box-Binding Transcription Factor; TGGCA-Binding Protein; Nuclear Factor I/B; Nuclear Factor 1/B; NF-I/B; NF1-B; NFI-B;
- Gene ID
- 4781
- UniProt ID
- O00712
- Long Name
- Nuclear Factor I B
- Sequence Similarities
- Belongs to the CTF/NF-I family.
- Cellular Localization
- Nucleus
- Protein Refseq
- NP_001177666.1; NP_001177667.1; NP_001269716.1; NP_001356387.1; NP_001356388.1
- Function
- Transcriptional activator of GFAP, essential for proper brain development.
Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "NFIB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1085-CN | Recombinant Mouse Anti-NFIB Monoclonal Antibody (1H2) | WB, IHC, IF, FC | Mouse IgG2a |
| MRO-1086-CN | Recombinant Mouse Anti-NFIB Monoclonal Antibody (1H4) | WB, IHC, FC | Mouse IgG1 |
| MRO-1087-CN | Recombinant Mouse Anti-NFIB Monoclonal Antibody (1H5) | WB, IHC, FC | Mouse IgG2b |
| MRO-1088-CN | Recombinant Mouse Anti-NFIB Monoclonal Antibody (1H1) | IHC, FC | Mouse IgG2b |
| MRO-2069-CN | Rabbit Anti-NFIB Polyclonal Antibody (MRO-2069-CN) | WB, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4813 | Anti-NFIB Immunohistochemistry Kit | IHC | |
| VS-0525-XY4814 | Anti-Mouse NFIB Immunohistochemistry Kit | IHC |
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