Mouse Anti-NFKBIA Recombinant Antibody (clone 4D4F2) (CAT#: VS7-0425-WR479)
This product is a mouse IgG1 antibody that binds to human NFKBIA. The antibody 4D4F2 recognizes specific amino acid domain of human NFKBIA (AA: 150-291). It can be used for ICC and FC assays.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/if_selected.jpg
Figure 2 IHC staining of human appendix
(Immunohistochemical staining of human appendix shows moderate cytoplasmic positivity in lymphoid cells and glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29207/62309_A_1_2_selected.jpg
(Immunohistochemical staining of human appendix shows moderate cytoplasmic positivity in lymphoid cells and glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29207/62309_A_1_2_selected.jpg
Figure 3 IHC staining of human heart muscle
(Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/ihc_selected.jpg
(Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/ihc_selected.jpg
Figure 4 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_A_8_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_A_8_8.jpg
Figure 5 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_B_8_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_B_8_5.jpg
Figure 6 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_A_8_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_A_8_3.jpg
Figure 7 Urinary bladder
(Urothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_A_6_5.jpg
(Urothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_A_6_5.jpg
Figure 8 RNA cell line category: Cell line enhanced (RT4)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100906-NFKBIA
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100906-NFKBIA
Specifications
- Immunogen
- Recombinant human NFKBIA (AA: 150-291) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human NFKBIA
- Species Reactivity
- Human
- Clone
- 4D4F2
- Molecule Class
- NF-κB Inhibitor Family
- Applications
- ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Ectodermal dysplasia and immunodeficiency 2 (EDAID2)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- NFKBIA; MAD3; Major Histocompatibility Complex Enhancer-Binding Protein MAD3; IkappaBalpha; IkB-Alpha; I-Kappa-B-Alpha; NF-Kappa-B Inhibitor Alpha; Nuclear Factor Of Kappa Light Chain Gene Enhancer In B-Cells; Nuclear Factor Of Kappa Light Polypeptide Gen
- Gene ID
- 4792
- UniProt ID
- P25963
- Long Name
- NFKB Inhibitor Alpha
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Hydroxylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_065390.1
- Function
- Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals (PubMed:1493333, PubMed:36651806, PubMed:7479976).; On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (PubMed:7479976, PubMed:7628694, PubMed:7796813, PubMed:7878466).
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Related Resources
TNF Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
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| MOR-2435 | Hi-Affi™ Recombinant Rabbit Anti-NFKBIA Monoclonal Antibody (DS2435AB) | ICC, IP, WB | IgG |
| MOR-4603 | Hi-Affi™ Recombinant Rabbit Anti-NFKBIA Monoclonal Antibody (TH116DS) | WB, IHC-P | IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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