Mouse Anti-NFKBIA Recombinant Antibody (clone 4D4F2)
CAT#: VS7-0425-WR479
This product is a mouse IgG1 antibody that binds to human NFKBIA. The antibody 4D4F2 recognizes specific amino acid domain of human NFKBIA (AA: 150-291). It can be used for ICC and FC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human appendix
Immunohistochemical staining of human appendix shows moderate cytoplasmic positivity in lymphoid cells and glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29207/62309_A_1_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human heart muscle
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/ihc_selected.jpg
Normal Tissue
Figure 4 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_A_8_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_A_8_3.jpg
Normal Tissue
Figure 7 Urinary bladder
Urothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_A_6_5.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (RT4)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100906-NFKBIA
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Specifications
- Immunogen
- Recombinant human NFKBIA (AA: 150-291) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human NFKBIA
- Species Reactivity
- Human
- Clone
- 4D4F2
- Molecule Class
- NF-κB Inhibitor Family
- Applications
- ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Ectodermal dysplasia and immunodeficiency 2 (EDAID2)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- NFKBIA; MAD3; Major Histocompatibility Complex Enhancer-Binding Protein MAD3; IkappaBalpha; IkB-Alpha; I-Kappa-B-Alpha; NF-Kappa-B Inhibitor Alpha; Nuclear Factor Of Kappa Light Chain Gene Enhancer In B-Cells; Nuclear Factor Of Kappa Light Polypeptide Gen
- Gene ID
- 4792
- UniProt ID
- P25963
- Long Name
- NFKB Inhibitor Alpha
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Hydroxylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_065390.1
- Function
- Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals (PubMed:1493333, PubMed:36651806, PubMed:7479976).; On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (PubMed:7479976, PubMed:7628694, PubMed:7796813, PubMed:7878466).
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Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
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- Product Name
Secondary Antibody
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Reconstitution Buffer
- CAT
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Recommended Dilution Buffer
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- Product Name
See other products for "NFKBIA"
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| VS-0424-XY202 | AbPlus™ Anti-NFKBIA Magnetic Beads (CBACN-302) | IP, Protein Purification | |
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| CAT | Product Name | Application | Type |
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