Rabbit Anti-NFKBIA Recombinant Antibody (VS13-YC799) (CAT#: VS13-YC799)
The Rabbit Anti-NFKBIA Recombinant Antibody is specific for human, mouse and rat NFKBIA. NFKBIA encodes an NF-kappa-B inhibitor with ankyrin repeat domains, interacting with REL dimers to inhibit NF-kappa-B/REL complexes. It shuttles between cytoplasm and nucleus and mutations can cause ectodermal dysplasia with immunodeficiency, with splice variants affecting its function. The anti-NFKBIA antibody can be used for Western blot (1/500-1/1000), Immunohistochemistry (1/50-1/100), Immunoprecipitation (1/20).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/if_selected.jpg
Figure 2 IHC staining of human appendix
(Immunohistochemical staining of human appendix shows moderate cytoplasmic positivity in lymphoid cells and glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29207/62309_A_1_2_selected.jpg
(Immunohistochemical staining of human appendix shows moderate cytoplasmic positivity in lymphoid cells and glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29207/62309_A_1_2_selected.jpg
Figure 3 IHC staining of human heart muscle
(Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/ihc_selected.jpg
(Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/ihc_selected.jpg
Figure 4 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_A_8_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_A_8_8.jpg
Figure 5 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_B_8_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_B_8_5.jpg
Figure 6 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_A_8_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_A_8_3.jpg
Figure 7 Urinary bladder
(Urothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_A_6_5.jpg
(Urothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/10280_A_6_5.jpg
Figure 8 RNA cell line category: Cell line enhanced (RT4)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100906-NFKBIA
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100906-NFKBIA
Specifications
- Immunogen
- Recombinant human IKB alpha.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat NFKBIA
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, IP
- Conjugate
- NONE
- Related Disease
- T-cell immunodeficiency autosomal dominant disease
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4) containing 150 mM NaCl, 40% Glycerol and 0.05% BSA.
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry (IHC): 1/50-1/100
Immunoprecipitation (IP): 1/20
Target
- Alternative Names
- NFKB Inhibitor Alpha; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor, Alpha; Major Histocompatibility Complex Enhancer-Binding Protein MAD3; NF-Kappa-B Inhibitor Alpha; I-Kappa-B-Alpha; IkappaBalpha; IkB-Alpha;
- Gene ID
- 4792
- UniProt ID
- P25963
- Long Name
- NFKB Inhibitor Alpha
- Sequence Similarities
- Belongs to the NF-kappa-B inhibitor family.
- Cellular Localization
- Cytoplasm; Nucleus
- Protein Refseq
- NP_065390.1
- Function
- Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Recommended Products
Secondary Antibody
Isotype Control
Related Resources
TNF Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1106MZ | Recombinant Mouse Anti-Human NFKBIA Antibody (clone 6A055) | WB | Mouse antibody |
| MRO-0822-CN | Recombinant Rabbit Anti-NFKBIA Monoclonal Antibody (CBACN-302) | WB, IF, IHC, IP | Rabbit IgG |
| MRO-2339-CN | Recombinant Rabbit Anti-NFKBIA (phosphorylated Ser32) Monoclonal Antibody (CBACN-630) | WB, IF, IP | Rabbit IgG |
| VS3-FY1036 | Recombinant Rabbit Anti-NFKBIA (phospho Ser36) Antibody (clone R05-4K3) | WB | Rabbit IgG |
| VS3-FY1037 | Recombinant Rabbit Anti-NFKBIA Antibody (clone R06-9E9) | WB | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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