Mouse Anti-NFKBIA Recombinant Antibody (VS3-CJ826)
CAT#: VS3-CJ826
This product is a mouse antibody that recognizes human, and mouse NFKBIA.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3815/if_selected.jpg
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Specifications
- Immunogen
- Purified His-tagged NFKBIA protein
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human, Mouse NFKBIA
- Species Reactivity
- Human, Mouse
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.3.
- Preservative
- 0.09% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:1000).
Target
- Alternative Names
- IKBA; MAD-3; NFKBI; EDAID2
- Gene ID
- 4792
- UniProt ID
- P25963
- Sequence Similarities
- Belongs to the NF-kappa-B inhibitor family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.
- Protein Refseq
- NP_065390.1
- Function
- Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
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Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "NFKBIA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2435 | Hi-Affi™ Recombinant Rabbit Anti-NFKBIA Monoclonal Antibody (DS2435AB) | ICC, IP, WB | IgG |
| MOR-4603 | Hi-Affi™ Recombinant Rabbit Anti-NFKBIA Monoclonal Antibody (TH116DS) | WB, IHC-P | IgG |
| MOR-4604 | Hi-Affi™ Recombinant Rabbit Anti-NFKBIA Monoclonal Antibody (TH117DS) | WB | IgG |
| MOR-4686 | Hi-Affi™ Recombinant Rabbit Anti-NFKBIA Monoclonal Antibody (TH200DS) | WB, FC | IgG |
| MOR-0076-FY | Rabbit Anti-NFKBIA Recombinant Antibody (clone AFY0047) | IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0822-CN | Recombinant Rabbit Anti-NFKBIA Monoclonal Antibody (CBACN-302) | WB, IF, IHC, IP | Rabbit IgG |
| ZG-0514J | Mouse Anti-NFKBIA Recombinant Antibody (ZG-0514J) | WB | Mouse IgG |
| ZG-0485U | Rabbit Anti-Phospho-NFKBIA (S32) Recombinant Antibody (clone 2D6) | ELISA, WB, IF | Rabbit IgG |
| ZG-0486U | Rabbit Anti-NFKBIA Recombinant Antibody (clone 1E9) | ELISA, WB, IHC, IP | Rabbit IgG |
| VS3-QX812 | Mouse Anti-NFKBIA Recombinant Antibody (clone 1A10) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY202 | AbPlus™ Anti-NFKBIA Magnetic Beads (CBACN-302) | IP, Protein Purification | |
| VS-0724-YC1191 | AbPlus™ Anti-NFKBIA Magnetic Beads (VS-0724-YC1191) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1496 | Anti-NFKBIA Immunohistochemistry Kit | IHC | |
| VS-0525-XY4821 | Anti-Mouse NFKBIA Immunohistochemistry Kit | IHC | |
| VS-0525-XY4820 | Anti-Human NFKBIA Immunohistochemistry Kit | IHC | |
| VS-0525-XY4822 | Anti-Rat NFKBIA Immunohistochemistry Kit | IHC |
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