Mouse Anti-NMI Recombinant Antibody (VS3-FY2822)
CAT#: VS3-FY2822
This anti-NMI product has been developed to target human NMI protein. It cross-reacts with mouse species. The calculated molecular weight of the protein is about 35.1 kDa. This monoclonal antibody is suitable for employment in Western Blot (1/500-1/2000), Immunocytochemistry (1/10-1/25) and Flow cytometry (1/10-1/25).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8588/42_G9_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human gallbladder
Immunohistochemical staining of human gallbladder shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8588/23758_A_6_4_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8588/23758_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8588/23758_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8588/23758_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8588/23758_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Preleptotene spermatocytes Staining: Low Intensity: Moderate Sertoli cells Staining: Low Intensity: Moderate Spermatogonia cells Staining: Low Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8588/23758_A_5_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8588/23758_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (NB-4)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000123609-NMI
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human and Mouse NMI
- Species Reactivity
- Human, Mouse
- Applications
- Western Blot, Immunocytochemistry, Flow cytometry
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 35.1 kDa.
- Related Disease
- Mouth Disease
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunocytochemistry: 1/10-1/25
Flow cytometry: 1/10-1/25
Target
- Alternative Names
- N-Myc And STAT Interactor; N-Myc (And STAT) Interactor; N-Myc-Interactor; NMI
- Gene ID
- 9111
- UniProt ID
- Q13287
- Sequence Similarities
- Belongs to the NMI family.
- Cellular Localization
- Cytoplasm
Nucleus
Secreted
- Post Translation Modifications
- Ubiquitinated.
'Lys-63'-linked ubiquitination by TRIM21 promotes interaction with IFI35 and inhibits virus-triggered type I IFN-beta production.
(Q13287-NMI_HUMAN)
Ubiquitination at Lys22, Lys70, Lys95, Lys102, Lys111, Lys129, Lys183, Lys245, Lys284, and Lys294 (NX_Q13287 [NX_Q13287-1])
Modification sites at PhosphoSitePlus (Q13287)
Glycosylation from GlyGen (Q13287) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_047302226.1; XP_054200448.1; XP_054200449.1; NP_004679.2
- Function
- Acts as a signaling pathway regulator involved in innate immune system response (PubMed:9989503, 26342464, 29038465, 29350881).
In response to interleukin 2/IL2 and interferon IFN-gamma/IFNG, interacts with signal transducer and activator of transcription/STAT which activate the transcription of downstream genes involved in a multitude of signals for development and homeostasis.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "NMI"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2231MZ | Recombinant Mouse Anti-Human NMI Antibody | IHC-P, WB | Mouse antibody |
| VS3-WK927 | Mouse Anti-NMI Recombinant Antibody (VS3-WK927) | WB, FC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2459 | Hi-Affi™ Recombinant Rabbit Anti-NMI Monoclonal Antibody (DS2459AB) | WB, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4879 | Anti-NMI Immunohistochemistry Kit | IHC |
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