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    Mouse Anti-NMI Recombinant Antibody (VS3-FY2822) (CAT#: VS3-FY2822)

    This anti-NMI product has been developed to target human NMI protein. It cross-reacts with mouse species. The calculated molecular weight of the protein is about 35.1 kDa. This monoclonal antibody is suitable for employment in Western Blot (1/500-1/2000), Immunocytochemistry (1/10-1/25) and Flow cytometry (1/10-1/25).

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    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

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    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line A-431
    Figure 1 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/8588/42_G9_2_selected.jpg
    Normal Tissue
    Figure 2 IHC staining of human gallbladder
    Figure 2 IHC staining of human gallbladder
    (Immunohistochemical staining of human gallbladder shows moderate cytoplasmic positivity in glandular cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/8588/23758_A_6_4_selected.jpg
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/8588/23758_B_9_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/8588/23758_A_9_3.jpg
    Figure 5 Liver
    Figure 5 Liver
    (Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/8588/23758_A_9_4.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/8588/23758_A_7_5.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Preleptotene spermatocytes Staining: Low Intensity: Moderate Sertoli cells Staining: Low Intensity: Moderate Spermatogonia cells Staining: Low Intensity: Moderate)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/8588/23758_A_5_6.jpg
    Figure 8 Lymph node
    Figure 8 Lymph node
    (Germinal center cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/8588/23758_A_9_8.jpg
    RNA Expression
    Figure 9 RNA cell line category: Cell line enhanced (NB-4)
    Figure 9 RNA cell line category: Cell line enhanced (NB-4)
    (Cell lines ordered by descending RNA expression order)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000123609-NMI

    Specifications

    • Host Species
    • Mouse
    • Type
    • Mouse IgG
    • Specificity
    • Human and Mouse NMI
    • Species Reactivity
    • Human, Mouse
    • Applications
    • Western Blot, Immunocytochemistry, Flow cytometry
    • Conjugate
    • Unconjugated, FITC
    • MW
    • The molecular weight of the target protein is approximately 35.1 kDa.
    • Related Disease
    • Mouth Disease

    Product Property

    • Clonality
    • Monoclonal Antibody
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • Western Blot: 1/500-1/2000
      Immunocytochemistry: 1/10-1/25
      Flow cytometry: 1/10-1/25

    Target

    • Alternative Names
    • N-Myc And STAT Interactor; N-Myc (And STAT) Interactor; N-Myc-Interactor; NMI
    • Sequence Similarities
    • Belongs to the NMI family.
    • Cellular Localization
    • Cytoplasm
      Nucleus
      Secreted
    • Post Translation Modifications
    • Ubiquitinated.
      'Lys-63'-linked ubiquitination by TRIM21 promotes interaction with IFI35 and inhibits virus-triggered type I IFN-beta production.
      (Q13287-NMI_HUMAN)
      Ubiquitination at Lys22, Lys70, Lys95, Lys102, Lys111, Lys129, Lys183, Lys245, Lys284, and Lys294 (NX_Q13287 [NX_Q13287-1])
      Modification sites at PhosphoSitePlus (Q13287)
      Glycosylation from GlyGen (Q13287) 1 site, 1 O-linked glycan (1 site)
    • Function
    • Acts as a signaling pathway regulator involved in innate immune system response (PubMed:9989503, 26342464, 29038465, 29350881).
      In response to interleukin 2/IL2 and interferon IFN-gamma/IFNG, interacts with signal transducer and activator of transcription/STAT which activate the transcription of downstream genes involved in a multitude of signals for development and homeostasis.

    Recommended Products

    Secondary Antibody

    Isotype Control

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "NMI"

    Select a product category from the dropdown menu below to view related products.
    Please select product type
    IgG Antibody Products Rabbit Monoclonal Antibody Products
    CAT Product Name Application Type
    MOB-2231MZ Recombinant Mouse Anti-Human NMI Antibody IHC-P, WB Mouse antibody
    VS3-WK927 Mouse Anti-NMI Recombinant Antibody (VS3-WK927) WB, FC Mouse IgG

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