Mouse Anti-NPPA Recombinant Antibody (VS3-CJ238)
CAT#: VS3-CJ238
This product is a mouse antibody that recognizes NPPA.
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58269/133550_B_7_5.jpg
Normal Tissue
Figure 2 Kidney
Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58269/133550_A_8_5.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58269/133550_A_6_6.jpg
Normal Tissue
Figure 4 High expression in heart muscle
Cardiomyocytes Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58269/133550_B_4_6.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (SCLC-21H, SH-SY5Y)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175206-NPPA
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Specifications
- Immunogen
- Recombinant protein of human NPPA
- Host Species
- Mouse
- Specificity
- Mouse NPPA
- Species Reactivity
- Mouse
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:2000).
Target
- Alternative Names
- Natriuretic peptides A; CDD-ANF; Cardiodilatin; CDD; Cardiodilatin-related peptide; CDP; Prepronatriodilatin; Atrial natriuretic factor; ANF; Atrial natriuretic peptide; ANP; NPPA; ANP; PND
- Gene ID
- 230899
- UniProt ID
- P01160
- Sequence Similarities
- Belongs to the natriuretic peptide family.
- Cellular Localization
- Cell projection, Secreted
- Post Translation Modifications
- The precursor molecule is proteolytically cleaved by CORIN at Arg-123 to produce atrial natriuretic peptide (PubMed:10880574, PubMed:14559895, PubMed:7984506).
Undergoes further proteolytic cleavage by unknown proteases to give rise to long-acting natriuretic peptide, vessel dilator and kaliuretic peptide (PubMed:2532366, PubMed:7955907, PubMed:7984506).
Additional processing gives rise to the auriculin and atriopeptin peptides (By similarity).
In the kidneys, alternative processing by an unknown protease results in the peptide urodilatin (PubMed:2972874, PubMed:8351194, PubMed:9794555).
Atrial natriuretic peptide:Cleavage by MME initiates degradation of the factor and thereby regulates its activity (PubMed:2972276, PubMed:16254193).
Degraded by IDE (in vitro) (PubMed:21098034).
During IDE degradation, the resulting products can temporarily stimulate NPR2 to produce cGMP, before the fragments are completely degraded and inactivated by IDE (in vitro) (PubMed:21098034).
Urodilatin: Degraded by IDE.
Urodilatin: Phosphorylation on Ser-129 decreases vasorelaxant activity.
- Protein Refseq
- NP_006163.1
- Function
- Atrial natriuretic peptide
Hormone that plays a key role in mediating cardio-renal homeostasis, and is involved in vascular remodeling and regulating energy metabolism (PubMed:8653797, PubMed:7595132, PubMed:2825692, PubMed:7720651, PubMed:8087923, PubMed:2532366, PubMed:22307324, PubMed:18835931, PubMed:21672517, PubMed:15741263, PubMed:16875975).
Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins, such as PRKG1, that drive various biological responses (PubMed:25401746, PubMed:9893117, PubMed:1672777, PubMed:1660465, PubMed:2162527, PubMed:2825692, PubMed:7720651, PubMed:22307324, PubMed:8384600, PubMed:21098034).
Regulates vasodilation, natriuresis, diuresis and aldosterone synthesis and is therefore essential for regulating blood pressure, controlling the extracellular fluid volume and maintaining the fluid-electrolyte balance (PubMed:8653797, PubMed:7595132, PubMed:2825692, PubMed:7720651, PubMed:2532366, PubMed:8087923).
Also involved in inhibiting cardiac remodeling and cardiac hypertrophy by inducing cardiomyocyte apoptosis and attenuating the growth of cardiomyocytes and fibroblasts (PubMed:16875975).
Plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus, and thus prevents pregnancy-induced hypertension (By similarity).
In adipose tissue, acts in various cGMP- and PKG-dependent pathways to regulate lipid metabolism and energy homeostasis (PubMed:22307324, PubMed:18835931, PubMed:21672517, PubMed:15741263).
This includes up-regulating lipid metabolism and mitochondrial oxygen utilization by activating the AMP-activated protein kinase (AMPK), and increasing energy expenditure by acting via MAPK11 to promote the UCP1-dependent thermogenesis of brown adipose tissue (PubMed:22307324, PubMed:18835931, PubMed:21672517, PubMed:15741263).
Binds the clearance receptor NPR3 which removes the hormone from circulation (PubMed:1672777).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "NPPA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2258z | Mouse Anti-NPPA Recombinant Antibody (clone 37B5) | WB, ELISA, IF, IP | Mouse IgG1, κ |
| MOB-0028MZ | Recombinant Mouse Anti-Human NPPA (amino acids 12-25) Antibody (clone CDI693) | ELISA | Mouse antibody |
| MRO-0102-CN | Recombinant Rabbit Anti-NPPA Monoclonal Antibody (JM08-36) | WB, IP | Rabbit IgG |
| ZG-0488U | Rabbit Anti-NPPA Recombinant Antibody (clone 2E3) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4924 | Anti-NPPA Immunohistochemistry Kit | IHC |
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