Mouse Anti-NPPA Recombinant Antibody (VS3-CJ238) (CAT#: VS3-CJ238)

• Immunogen
• Recombinant protein of human NPPA

• Host Species
• Mouse

• Specificity
• Mouse NPPA

• Species Reactivity
• Mouse

• Applications
• WB

• Conjugate
• Unconjugated

• Purification
• Affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• PBS

• Preservative
• 0.09% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in Western Blot (1:2000).

• Alternative Names
• Natriuretic peptides A; CDD-ANF; Cardiodilatin; CDD; Cardiodilatin-related peptide; CDP; Prepronatriodilatin; Atrial natriuretic factor; ANF; Atrial natriuretic peptide; ANP; NPPA; ANP; PND

• Gene ID
• 230899

• UniProt ID
• P01160

• Sequence Similarities
• Belongs to the natriuretic peptide family.

• Cellular Localization
• Cell projection, Secreted

• Post Translation Modifications
• The precursor molecule is proteolytically cleaved by CORIN at Arg-123 to produce atrial natriuretic peptide (PubMed:10880574, PubMed:14559895, PubMed:7984506).
  Undergoes further proteolytic cleavage by unknown proteases to give rise to long-acting natriuretic peptide, vessel dilator and kaliuretic peptide (PubMed:2532366, PubMed:7955907, PubMed:7984506).
  Additional processing gives rise to the auriculin and atriopeptin peptides (By similarity).
  In the kidneys, alternative processing by an unknown protease results in the peptide urodilatin (PubMed:2972874, PubMed:8351194, PubMed:9794555).
  Atrial natriuretic peptide:Cleavage by MME initiates degradation of the factor and thereby regulates its activity (PubMed:2972276, PubMed:16254193).
  Degraded by IDE (in vitro) (PubMed:21098034).
  During IDE degradation, the resulting products can temporarily stimulate NPR2 to produce cGMP, before the fragments are completely degraded and inactivated by IDE (in vitro) (PubMed:21098034).
  Urodilatin: Degraded by IDE.
  Urodilatin: Phosphorylation on Ser-129 decreases vasorelaxant activity.

• Protein Refseq
• NP_006163.1

• Function
• Atrial natriuretic peptide
  Hormone that plays a key role in mediating cardio-renal homeostasis, and is involved in vascular remodeling and regulating energy metabolism (PubMed:8653797, PubMed:7595132, PubMed:2825692, PubMed:7720651, PubMed:8087923, PubMed:2532366, PubMed:22307324, PubMed:18835931, PubMed:21672517, PubMed:15741263, PubMed:16875975).
  Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins, such as PRKG1, that drive various biological responses (PubMed:25401746, PubMed:9893117, PubMed:1672777, PubMed:1660465, PubMed:2162527, PubMed:2825692, PubMed:7720651, PubMed:22307324, PubMed:8384600, PubMed:21098034).
  Regulates vasodilation, natriuresis, diuresis and aldosterone synthesis and is therefore essential for regulating blood pressure, controlling the extracellular fluid volume and maintaining the fluid-electrolyte balance (PubMed:8653797, PubMed:7595132, PubMed:2825692, PubMed:7720651, PubMed:2532366, PubMed:8087923).
  Also involved in inhibiting cardiac remodeling and cardiac hypertrophy by inducing cardiomyocyte apoptosis and attenuating the growth of cardiomyocytes and fibroblasts (PubMed:16875975).
  Plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus, and thus prevents pregnancy-induced hypertension (By similarity).
  In adipose tissue, acts in various cGMP- and PKG-dependent pathways to regulate lipid metabolism and energy homeostasis (PubMed:22307324, PubMed:18835931, PubMed:21672517, PubMed:15741263).
  This includes up-regulating lipid metabolism and mitochondrial oxygen utilization by activating the AMP-activated protein kinase (AMPK), and increasing energy expenditure by acting via MAPK11 to promote the UCP1-dependent thermogenesis of brown adipose tissue (PubMed:22307324, PubMed:18835931, PubMed:21672517, PubMed:15741263).
  Binds the clearance receptor NPR3 which removes the hormone from circulation (PubMed:1672777).

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS