Rabbit Anti-NRAS Recombinant Antibody (clone R01-7D0)
CAT#: VS3-FY2077
The R01-7D0 recombinant monoclonal antibody exhibits specific binding to human NRAS. It cross-reacts with mouse, rat, hamster. It is suitable for use in a variety of assays, including WB, IP. The antibody has the positive detection of GTPase HRAS by western blot analysis of GTPase HRAS in 3T3, mouse brain lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79912/1987_E10_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10157/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_A_9_3.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000213281-NRAS
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WB
Figure 1 Anti-NRAS Antibody (VS3-FY2077) in WB.
The GTPase HRAS expression in 3T3, mouse brain lysates were detected using the anti-GTPase HRAS by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human Ras
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat, Hamster NRAS
- Species Reactivity
- Human, Mouse, Rat, Hamster
- Clone
- R01-7D0
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 21 kDa, while the observed molecular weight stands at 21 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- NRAS Proto-Oncogene, GTPase; Neuroblastoma RAS Viral (V-Ras) Oncogene Homolog; Neuroblastoma RAS Viral Oncogene Homolog; Transforming Protein N-Ras; V-Ras Neuroblastoma RAS Viral Oncogene Homolog; N-Ras Protein Part 4; GTPase NRas; HRAS1
- Gene ID
- 4893
- UniProt ID
- P01111
- Cellular Localization
- Cell membrane, Golgi apparatus, Membrane
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NRAS"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1454CT | Recombinant Mouse anti-Human NRAS Monoclonal antibody (BU3H0) | ELISA, WB | |
| VS3-CJ234 | Mouse Anti-NRAS Recombinant Antibody (VS3-CJ234) | WB | Mouse IgG1 |
| VS3-CJ235 | Rabbit Anti-NRAS Recombinant Antibody (VS3-CJ235) | WB, IP, FC | Rabbit IgG |
| VS3-FY1069 | Recombinant Rabbit Anti-NRAS Antibody (clone R09-7E8) | WB, ICC, IF, IP | Rabbit IgG |
| VS13-YC842 | Rabbit Anti-NRAS Recombinant Antibody (VS13-YC842) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2498 | Hi-Affi™ Recombinant Rabbit Anti-NRAS Monoclonal Antibody (DS2498AB) | WB, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF595 | A*01:01/Human N-RAS (ILDTAGREEY) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY205 | AbPlus™ Anti-NRAS Magnetic Beads (JF10-11) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1518 | Anti-NRAS Immunohistochemistry Kit | IHC | |
| VS-0525-XY4973 | Anti-Mouse NRAS Immunohistochemistry Kit | IHC | |
| VS-0525-XY4972 | Anti-Human NRAS Immunohistochemistry Kit | IHC |
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