Rabbit Anti-ORAI3 Recombinant Antibody (clone R06-8B3)
CAT#: VS3-FY2055
This recombinant antibody is positive verification in western blot analysis of orai3 in Hela, A549, HL-60, U251, U87-MG lysates. It is a rabbit recombinant monoclonal antibody that specifically immunoreacts with human ORAI3. It is suitable for use in a variety of assays, including WB, IHC-P. This R06-8B3 antibody is validated for western blot analysis of Orai3 in Hela, A549, HL-60, U251, U87-MG lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15022/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15022/35497_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15022/35497_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15022/35497_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15022/35497_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15022/35497_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15022/35497_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (HHSteC)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175938-ORAI3
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WB
Figure 1 Anti-ORAI3 Antibody (VS3-FY2055) in WB.
The Orai3 expression in Hela, A549, HL-60, U251, U87-MG lysates were detected using the anti-ORAI3 by western blot assay.
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Specifications
- Immunogen
- Recombinant human Orai3 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ORAI3
- Species Reactivity
- Human
- Clone
- R06-8B3
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 31 kDa, while the observed molecular weight stands at 31 kDa.
- Related Disease
- Immunodeficiency 10 and T Cell And Nk Cell Immunodeficiency
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- ORAI Calcium Release-Activated Calcium Modulator 3; Transmembrane Protein 142C; TMEM142C; Protein Orai-3
- Gene ID
- 93129
- UniProt ID
- Q9BRQ5
- Sequence Similarities
- Belongs to the Orai family.
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Glycosylation at Ser213, Ser214, Ser216, and Ser220 (NX_Q9BRQ5 [NX_Q9BRQ5-1])
Modification sites at PhosphoSitePlus (Q9BRQ5)
Glycosylation from GlyGen (Q9BRQ5) 6 sites, 2 O-linked glycans (6 sites)
- Protein Refseq
- NP_689501.1
- Function
- Ca²⁺ release-activated Ca²⁺-like (CRAC-like) channel subunit which mediates Ca²⁺ influx and increase in Ca²⁺-selective current by synergy with the Ca²⁺ sensor, STIM1.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ORAI3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2097-CN | Rabbit Anti-ORAI3 Polyclonal Antibody (MRO-2097-CN) | WB, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5099 | Anti-ORAI3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5100 | Anti-Rat ORAI3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5101 | Anti-Mouse ORAI3 Immunohistochemistry Kit | IHC |
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