Rabbit Anti-ORAI3 Recombinant Antibody (clone R06-8B3) (CAT#: VS3-FY2055)
This recombinant antibody is positive verification in western blot analysis of orai3 in Hela, A549, HL-60, U251, U87-MG lysates. It is a rabbit recombinant monoclonal antibody that specifically immunoreacts with human ORAI3. It is suitable for use in a variety of assays, including WB, IHC-P. This R06-8B3 antibody is validated for western blot analysis of Orai3 in Hela, A549, HL-60, U251, U87-MG lysates.
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WB
Figure 1 Anti-ORAI3 Antibody (VS3-FY2055) in WB.
The Orai3 expression in Hela, A549, HL-60, U251, U87-MG lysates were detected using the anti-ORAI3 by western blot assay.
Specifications
- Immunogen
- Recombinant human Orai3 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ORAI3
- Species Reactivity
- Human
- Clone
- R06-8B3
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 31 kDa, while the observed molecular weight stands at 31 kDa.
- Related Disease
- Immunodeficiency 10 and T Cell And Nk Cell Immunodeficiency
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- ORAI Calcium Release-Activated Calcium Modulator 3; Transmembrane Protein 142C; TMEM142C; Protein Orai-3
- Gene ID
- 93129
- UniProt ID
- Q9BRQ5
- Sequence Similarities
- Belongs to the Orai family.
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Glycosylation at Ser213, Ser214, Ser216, and Ser220 (NX_Q9BRQ5 [NX_Q9BRQ5-1])
Modification sites at PhosphoSitePlus (Q9BRQ5)
Glycosylation from GlyGen (Q9BRQ5) 6 sites, 2 O-linked glycans (6 sites)
- Protein Refseq
- NP_689501.1
- Function
- Ca²⁺ release-activated Ca²⁺-like (CRAC-like) channel subunit which mediates Ca²⁺ influx and increase in Ca²⁺-selective current by synergy with the Ca²⁺ sensor, STIM1.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2097-CN | Rabbit Anti-ORAI3 Polyclonal Antibody (MRO-2097-CN) | WB, IF, IHC, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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