Rabbit Anti-OTX1 Recombinant Antibody (clone R49-5O-3) (CAT#: VS3-FY2122)
Clone R49-5O-3 is a rabbit IgG recombinant monoclonal antibody that is specifically directed against human OTX1 protein, a calculated weight of 37 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R49-5O-3 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEL
(Immunofluorescent staining of human cell line HEL shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52122/1849_F10_32_selected.jpg
(Immunofluorescent staining of human cell line HEL shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52122/1849_F10_32_selected.jpg
Figure 2 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows distinct positivity in neuropil.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52122/ihc_selected.jpg
(Immunohistochemical staining of human cerebral cortex shows distinct positivity in neuropil.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52122/ihc_selected.jpg
Figure 3 Cerebral cortex
(Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52122/122679_B_8_5.jpg
(Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52122/122679_B_8_5.jpg
Figure 4 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52122/122679_A_6_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52122/122679_A_6_6.jpg
Figure 5 Skin
(Keratinocytes Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52122/122679_B_9_1.jpg
(Keratinocytes Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52122/122679_B_9_1.jpg
Figure 6 RNA cell line category: Cell line enriched (THP-1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000115507-OTX1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000115507-OTX1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat OTX1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R49-5O-3
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 37 kDa, while the observed molecular weight stands at 32kDa.
- Related Disease
- Epilepsy and Agnathia-Otocephaly Complex
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Target
- Alternative Names
- Orthodenticle Homeobox 1; Orthodenticle (Drosophila) Homolog 1; Orthodenticle Homolog 1 (Drosophila); Orthodenticle Homolog 1
- Gene ID
- 5013
- UniProt ID
- P32242
- Sequence Similarities
- Belongs to the paired homeobox family. Bicoid subfamily.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (P32242)
Modification sites at neXtProt (NX_P32242)
Glycosylation from GlyGen (P32242) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001186699.1; NP_055377.1
- Function
- Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "OTX1"
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1511CT | Recombinant Mouse anti-Human OTX1 Monoclonal antibody (EML2289) | WB | |
| VS3-FY2056 | Rabbit Anti-OTX1 Recombinant Antibody (clone R04-1D1) | WB | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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