Rabbit Anti-OTX1 Recombinant Antibody (clone R49-5O-3)
CAT#: VS3-FY2122
Clone R49-5O-3 is a rabbit IgG recombinant monoclonal antibody that is specifically directed against human OTX1 protein, a calculated weight of 37 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R49-5O-3 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52122/1849_F10_32_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows distinct positivity in neuropil.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52122/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52122/122679_B_8_5.jpg
Normal Tissue
Figure 4 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52122/122679_A_6_6.jpg
Normal Tissue
Figure 5 Skin
Keratinocytes Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52122/122679_B_9_1.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enriched (THP-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000115507-OTX1
❮
❯
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat OTX1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R49-5O-3
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 37 kDa, while the observed molecular weight stands at 32kDa.
- Related Disease
- Epilepsy and Agnathia-Otocephaly Complex
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Target
- Alternative Names
- Orthodenticle Homeobox 1; Orthodenticle (Drosophila) Homolog 1; Orthodenticle Homolog 1 (Drosophila); Orthodenticle Homolog 1
- Gene ID
- 5013
- UniProt ID
- P32242
- Sequence Similarities
- Belongs to the paired homeobox family. Bicoid subfamily.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (P32242)
Modification sites at neXtProt (NX_P32242)
Glycosylation from GlyGen (P32242) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001186699.1; NP_055377.1
- Function
- Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY2122. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "OTX1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1511CT | Recombinant Mouse anti-Human OTX1 Monoclonal antibody (EML2289) | WB | |
| VS3-FY2056 | Rabbit Anti-OTX1 Recombinant Antibody (clone R04-1D1) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5119 | Anti-OTX1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5120 | Anti-Mouse OTX1 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, IF, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, Inhib, FuncS
Application: ELISA, Neut
Application: ELISA, IHC, IF, IP, FC, FuncS
Application: ELISA, FC, Neut, Inhib
Application: ELISA, FC
Application: ELISA, Neut, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
