Rabbit Anti-PCBD1 Recombinant Antibody (clone R41-2R-5)
CAT#: VS3-FY2179
Clone R41-2R-5 is a rabbit recombinant monoclonal antibody that has the ability to specifically bind to human PCBD1 protein, a calculated weight of 12 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R41-2R-5 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37575/403_G5_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61723/if_selected.jpg
Normal Tissue
Figure 3 Liver
Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37575/74189_A_9_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37575/74189_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37575/74189_A_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (CACO-2, Hep G2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166228-PCBD1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PCBD1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R41-2R-5
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 12 kDa, while the observed molecular weight stands at 12kDa.
- Related Disease
- Hyperphenylalaninemia, Bh4-Deficient, D and Hyperphenylalaninemia
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Target
- Alternative Names
- Pterin-4 Alpha-Carbinolamine Dehydratase 1; -Pyruvoyl-Tetrahydropterin Synthase/Dimerization Cofactor Of Hepatocyte Nuclear Factor 1 Alpha (TCF1); Pterin-4 Alpha-Carbinolamine Dehydratase/Dimerization Cofactor Of Hepatocyte Nuclear Factor 1 Alpha; Phenylalanine Hydroxylase-Stimulating Protein; 4-Alpha-Hydroxy-Tetrahydropterin Dehydratase; Dimerizing Cofactor For HNF1; EC 4.2.1.96; DCOH; PCBD; PCD
- Gene ID
- 5092
- UniProt ID
- P61457
- Sequence Similarities
- Belongs to the pterin-4-alpha-carbinolamine dehydratase family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Ubiquitination at Lys41 and Lys59 (NX_P61457 [NX_P61457-1])
Modification sites at PhosphoSitePlus (P61457)
- Protein Refseq
- NP_000272.1; NP_001276726.1; NP_001309933.1
- Function
- Regulates the dimerization of homeodomain protein HNF1A and enhances its transcriptional activity (By similarity).
Also acts as a coactivator for HNF1B-dependent transcription.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PCBD1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1568CT | Recombinant Mouse anti-Human PCBD1 Monoclonal antibody (EML559) | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2609 | Hi-Affi™ Recombinant Rabbit Anti-PCBD1 Monoclonal Antibody (DS2609AB) | WB, IHC, ICC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1577 | Anti-PCBD1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5228 | Anti-Human PCBD1 Immunohistochemistry Kit | IHC |
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