Mouse Anti-PCRV Recombinant Antibody (clone m166) (CAT#: HPAB-2055-FY)
This product is a recombinant mouse antibody that recognizes PCRV. The monoclonal mouse antibody m166 reacts with the epitope of PCRV.
Specific Inquiry
Neut
Figure 1 In vivo screen for monoclonal antibodies (MAbs) that prevent sepsis in an acute lung infection model.
Survival measured for 48 h.
Frank, D. W., Vallis, A., Wiener-Kronish, J. P., Roy-Burman, A., Spack, E. G., Mullaney, B. P., ... & Sawa, T. (2002). Generation and characterization of a protective monoclonal antibody to Pseudomonas aeruginosa PcrV. The Journal of infectious diseases, 186(1), 64-73.
Neut
Figure 2 Efficacy of monoclonal antibody (MAb) 166 during a coinstillation and passive immunization.
Purified polyclonal (poly) rabbit (Rab) IgG, control preimmune rabbit IgG, mouse MAb 166, or irrelevant mouse IgG2bK (MOPC-141) premixed with a bacterial inoculum consisting of Pseudomonas aeruginosa strain PA103 ( cfu, 4-fold lethal dose). The mixture was instilled into the 2.0 x 106 lungs of individual mic, and survival was assessed for 7 days.
Frank, D. W., Vallis, A., Wiener-Kronish, J. P., Roy-Burman, A., Spack, E. G., Mullaney, B. P., ... & Sawa, T. (2002). Generation and characterization of a protective monoclonal antibody to Pseudomonas aeruginosa PcrV. The Journal of infectious diseases, 186(1), 64-73.
WB
Figure 3 Monoclonal antibody (MAb) 166 binds to the carboxylterminus of PcrV.
Bacterial lysates were prepared after induction and were analyzed for antibody binding by Western blot analysis. MAb 166 bound to full-length clones and a carboxyl-terminal expression clone but not to amino-terminal clones or an internal deletion protein.
Frank, D. W., Vallis, A., Wiener-Kronish, J. P., Roy-Burman, A., Spack, E. G., Mullaney, B. P., ... & Sawa, T. (2002). Generation and characterization of a protective monoclonal antibody to Pseudomonas aeruginosa PcrV. The Journal of infectious diseases, 186(1), 64-73.
ELISA
Figure 4 Binding affinities of Mab166 and scFv166 in competition ELISA.
Sawa, T., Kainuma, A., Moriyama, K., & Naito, Y. (2017). Construction and Characteristics of a Recombinant Single-Chain Antibody Fragment against Bacterial Type III Secretion. In Antibody Engineering. IntechOpen.
Specifications
- Immunogen
- Recombinant PcrV protein and native PcrV of P. aeruginosa PA103.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- PCRV
- Species Reactivity
- PCRV
- Clone
- m166
- Applications
- Neut, WB, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The PCRV antibody has been reported in applications of Neutralization, Western Blot, Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- P. aeruginosa V; PCRV
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone m166"
See other products for "PcrV"
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0950CL | Mouse Anti-PcrV Recombinant Antibody (TAB-0950CL) | Sandwich Immunoassay | Mouse IgG2b |
| TAB-0951CL | Mouse Anti-PcrV Recombinant Antibody (TAB-0951CL) | WB, ELISA, FuncS | Mouse IgG2a |
| TAB-0952CL | Mouse Anti-PcrV Recombinant Antibody (TAB-0952CL) | Sandwich Immunoassay | Mouse IgG2a |
| MRO-157MZ-F(E) | Anti-P. aeruginosa PcrV Recombinant Antibody Fab Fragment (MAb 179) (MRO-157MZ-F(E)) | WB, ELISA | |
| MRO-158MZ-F(E) | Anti-P. aeruginosa PcrV Recombinant Antibody Fab Fragment (MAb 203) (MRO-158MZ-F(E)) | WB, ELISA |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-472CQ | Anti-Pseudomonas Aeruginosa pcrV Recombinant Antibody Fab Fragment (Rivabazumab) | ELISA, IHC, FC, IP, IF, FuncS | Fab' G1-κ |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP217-YC | Llama Anti-PcrV Recombinant Single Domain Antibody (HPAB-AP217-YC) | ELISA, Inhib | Llama VHH |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP778-YC | Recombinant Human Anti-PcrV Antibody (Ab1) | ELISA, Neut | Human IgG |
| HPAB-AP779-YC | Recombinant Human Anti-PcrV Antibody (Ab2) | ELISA, Neut | Human IgG |
| HPAB-AP780-YC | Recombinant Human Anti-PcrV Antibody (Ab3) | ELISA, Neut | Human IgG |
| HPAB-J0211-YC | Recombinant Human Anti-PcrV Antibody (V2L2) | ELISA, FuncS | Human IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP778-YC-S(P) | Recombinant Human Anti-PcrV Antibody scFv Fragment (Ab1) | ELISA, Neut | Human scFv |
| HPAB-AP779-YC-S(P) | Recombinant Human Anti-PcrV Antibody scFv Fragment (Ab2) | ELISA, Neut | Human scFv |
| HPAB-AP780-YC-S(P) | Recombinant Human Anti-PcrV Antibody scFv Fragment (Ab3) | ELISA, Neut | Human scFv |
| HPAB-J0211-YC-S(P) | Recombinant Human Anti-PcrV Antibody scFv Fragment (V2L2) | ELISA, FuncS | Human scFv |
| HPAB-2055-FY-S(P) | Mouse Anti-PCRV Recombinant Antibody (clone m166); scFv Fragment | WB, ELISA | Mouse scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP778-YC--F(E) | Recombinant Human Anti-PcrV Antibody Fab Fragment (Ab1) | ELISA, Neut | Human Fab |
| HPAB-AP779-YC--F(E) | Recombinant Human Anti-PcrV Antibody Fab Fragment (Ab2) | ELISA, Neut | Human Fab |
| HPAB-AP780-YC--F(E) | Recombinant Human Anti-PcrV Antibody Fab Fragment (Ab3) | ELISA, Neut | Human Fab |
| HPAB-J0211-YC-F(E) | Recombinant Human Anti-PcrV Antibody Fab Fragment (V2L2) | ELISA, FuncS | Human Fab |
| HPAB-2055-FY-F(E) | Mouse Anti-PCRV Recombinant Antibody (clone m166); Fab Fragment | WB, ELISA | Mouse Fab |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for HPAB-2055-FY. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.