Rabbit Anti-PDLIM7 Recombinant Antibody (clone R05-5A1)
CAT#: VS3-FY1822
This anti-PDLIM7 antibody detected positive in western blot analysis of PDLIM7 in Hela lysates. The recombinant monoclonal antibody demonstrates specific reactivity with human PDLIM7. The R05-5A1 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000).
Tested Data
Gene Expression
WB
Figure 1 Anti-PDLIM7 Antibody (VS3-FY1822) in WB.
The PDLIM7 expression in Hela lysates were detected using the anti-PDLIM7 by western blot assay.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to actin filaments & focal adhesion sites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18794/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, actin filaments & focal adhesion sites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48815/751_E9_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human heart muscle
Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18794/44480_B_5_6_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18794/44480_B_7_5.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18794/44480_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18794/44480_A_5_6.jpg
Normal Tissue
Figure 7 Skeletal muscle
Myocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18794/44480_B_3_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196923-PDLIM7
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Specifications
- Immunogen
- Recombinant human PDLIM7 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PDLIM7
- Species Reactivity
- Human
- Clone
- R05-5A1
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 50 kDa, while the observed molecular weight stands at 50 kDa.
- Related Disease
- Lymphoepithelioma-Like Carcinoma and Hodgkin's Granuloma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- PDZ And LIM Domain 7; PDZ And LIM Domain 7 (Enigma); Protein Enigma; LMP; Lim Mineralization Protein 3; PDZ And LIM Domain Protein 7; LIM Mineralization Protein
- Gene ID
- 9260
- UniProt ID
- Q9NR12
- Cellular Localization
- Cytoplasm, Cytoskeleton
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q9NR12)
Modification sites at neXtProt (NX_Q9NR12)
Glycosylation from GlyConnect PDLI7_HUMAN (2908)
Glycosylation from GlyGen (Q9NR12) 11 sites, 3 O-linked glycans (11 sites)
- Protein Refseq
- NP_005442.2; NP_976227.1; NP_998801.1
- Function
- May function as a scaffold on which the coordinated assembly of proteins can occur. May play a role as an adapter that, via its PDZ domain, localizes LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PDLIM7"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1329CT | Recombinant Mouse anti-Human PDLIM7 Monoclonal antibody (EML931) | WB | |
| VS7-0425-WR589 | Mouse Anti-PDLIM7 Recombinant Antibody (clone 3B3B3) | IHC, ICC, FC | Mouse IgG1 |
| VS7-0425-WR590 | Mouse Anti-PDLIM7 Recombinant Antibody (clone 2B4A3) | IHC, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2648 | Hi-Affi™ Recombinant Rabbit Anti-PDLIM7 Monoclonal Antibody (DS2648AB) | IHC-P, IHC-Fr | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1610 | Anti-PDLIM7 Immunohistochemistry Kit | IHC |
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