Recombinant Mouse Anti-Pectinase Antibody (B1) (CAT#: FAMAB-0304YC)
The antibodies produced by individual clones inhibited polygalacturonase in the culture supernatants of a range of fungal pathogens, including ascomycetes, basidiomycetes, and oomycetes. The soluble antibodies also inhibited maceration of potato tissue by these pathogens.
Specific Inquiry
WB
Figure 1 Binding of recombinant ScFvs to extracellular proteins from different fungal species.
Western blots of proteins from culture supernatants were probed with the bacterial ScFvs B1 (lanes 1-8) and B4 (Lanes 9-16).
Manatunga, V. , Sanati, H. , Tan, P. , & Philip A. O’Brien. (2005). Maceration of plant tissue by fungi is inhibited by recombinant antipectinase antibodies. European Journal of Plant Pathology, 112(3), 211-220.
ELISA
Figure 2 Inhibition of fungal polygalacturonase activity by ScFv B1 and B4.
Increasing amounts of bacterial ScFv were added to a constant amount of enzyme.
Manatunga, V. , Sanati, H. , Tan, P. , & Philip A. O’Brien. (2005). Maceration of plant tissue by fungi is inhibited by recombinant antipectinase antibodies. European Journal of Plant Pathology, 112(3), 211-220.
Specifications
- Host Species
- Mouse
- Derivation
- Phage display library
- Type
- Mouse IgG
- Specificity
- Rhizoctonia solani Pectinase
- Species Reactivity
- Rhizoctonia solani
- Clone
- B1
- Applications
- ELISA, Western Blot
- Related Disease
- Fungal infection
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
Fab Antibody Products
ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-760 | Recombinant Anti-Pectinase Antibody | WB, IHC, FuncS | IgG |
| MHH-760 | Recombinant Human Anti-Pectinase Antibody | ELISA, WB, FuncS | IgG |
| FAMAB-0303YC | Recombinant Mouse Anti-Pectinase Antibody (B4) | ELISA, WB | Mouse IgG |
| FAMAB-1484CQ | Mouse Anti-Pectinase Recombinant Antibody (clone B1) | FuncS, WB, ELISA | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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