Rabbit Anti-PEF1 Recombinant Antibody (clone R09-7F3)
CAT#: VS3-FY1824
This R09-7F3 recombinant monoclonal antibody exhibits specific binding to human PEF1. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IP. This PEF1 antibody has been rigorously validated and is widely used in western blot analysis of PEF1 in K562, rat brain, C6, 3T3, Hela lysates.
Tested Data
Gene Expression
WB
Figure 1 Anti-PEF1 Antibody (VS3-FY1824) in WB.
The PEF1 expression in K562, rat Brain, C6, 3T3, Hela lysates were detected using the anti-PEF1 by western blot assay.
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Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm, cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61608/1210_C10_2_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000162517-PEF1
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Specifications
- Immunogen
- Synthetic peptide of human PEF1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PEF1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R09-7F3
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 30 kDa, while the observed molecular weight stands at 30 kDa.
- Related Disease
- Tyrosinemia, Type Iii
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Penta-EF-Hand Domain Containing 1; PEF Protein With A Long N-Terminal Hydrophobic Domain; Peflin; ABP32; Penta-EF Hand Domain-Containing Protein 1; PEF1A
- Gene ID
- 553115
- UniProt ID
- Q9UBV8
- Cellular Localization
- Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane
- Post Translation Modifications
- Ubiquitinated by the BCR(KLHL12) E3 ubiquitin ligase complex. (Q9UBV8-PEF1_HUMAN)
Ubiquitination at Lys137 (NX_Q9UBV8 [NX_Q9UBV8-1])
Modification sites at PhosphoSitePlus (Q9UBV8)
Glycosylation from GlyGen (Q9UBV8) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011540047.1; XP_011540048.1; XP_011540049.1; XP_016857169.1; XP_016857170.2
- Function
- It may act as a negative regulator of PDCD6.
Also acts as a negative regulator of ER-Golgi transport; possibly by inhibiting interaction between PDCD6 and SEC31 (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PEF1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2658 | Hi-Affi™ Recombinant Rabbit Anti-PEF1 Monoclonal Antibody (DS2658AB) | WB, IP, FC | IgG |
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