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    Rabbit Anti-PER3 Recombinant Antibody (clone R74-4J-9) (CAT#: VS3-FY2132)

    Clone R74-4J-9 is a rabbit recombinant monoclonal antibody that specifically directed against human PER3 protein, a calculated weight of 132 kDa. It cross-reacts with mouse, rat.
    The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R74-4J-9 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000).

    Specific Inquiry
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    • Conjugation:

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    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19530/if_selected.jpg
    Normal Tissue
    Figure 2 Cerebral cortex
    Figure 2 Cerebral cortex
    (Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19530/44115_B_8_5.jpg
    Figure 3 Colon
    Figure 3 Colon
    (Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19530/44115_A_9_3.jpg
    Figure 4 Liver
    Figure 4 Liver
    (Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19530/44115_A_9_4.jpg
    Figure 5 Kidney
    Figure 5 Kidney
    (Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19530/44115_A_8_5.jpg
    Figure 6 Testis
    Figure 6 Testis
    (Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19530/44115_A_4_6.jpg
    Figure 7 Lymph node
    Figure 7 Lymph node
    (Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19530/44115_A_9_8.jpg
    RNA Expression
    Figure 8 RNA cell line category: Cell line enhanced (U-937)
    Figure 8 RNA cell line category: Cell line enhanced (U-937)
    (Cell lines ordered by descending RNA expression order)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000049246-PER3

    Specifications

    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human, Mouse and Rat PER3
    • Species Reactivity
    • Human, Mouse, Rat
    • Clone
    • R74-4J-9
    • Applications
    • Western Blot
    • Conjugate
    • Unconjugated
    • MW
    • The calculated weight of the target protein is approximately 132kDa, while the observed molecular weight stands at 132kDa.
    • Related Disease
    • Advanced Sleep Phase Syndrome, Familial, 3 and Advanced Sleep Phase Syndrome

    Product Property

    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • PBS (pH 7.4), 150 mM NaCl, 50% glycerol
    • Preservative
    • 0.02% Sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • Western Blot: 1/500-1/1000

    Target

    • Alternative Names
    • Period Circadian Regulator 3; Cell Growth-Inhibiting Gene 13 Protein; Circadian Clock Protein PERIOD 3; Period Circadian Clock 3; HPER3; Period Circadian Protein Homolog 3
    • Cellular Localization
    • Cytoplasm, Nucleus
    • Post Translation Modifications
    • Phosphorylation by CSNK1E is weak and appears to require association with PER1 and translocation to the nucleus. (P56645-PER3_HUMAN)
      Ubiquitinated. (P56645-PER3_HUMAN)
      Modification sites at PhosphoSitePlus (P56645)
      Modification sites at neXtProt (NX_P56645)
      Glycosylation from GlyGen (P56645) 1 site, 1 O-linked glycan (1 site)
    • Function
    • It is derived from the Latin roots 'circa' (about) and 'diem' (day) and acts as an important regulator of a wide array of physiological functions including metabolism, sleep, body temperature, blood pressure, endocrine, immune, cardiovascular, and renal function. Consists of two major components: the central clock, residing in the suprachiasmatic nucleus (SCN) of the brain, and the peripheral clocks that are present in nearly every tissue and organ system. Both the central and peripheral clocks can be reset by environmental cues, also known as Zeitgebers.

    Recommended Products

    Isotype Control

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "PER3"

    Select a product category from the dropdown menu below to view related products.
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    Rabbit Monoclonal Antibody Products IgG Antibody Products IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
    CAT Product Name Application Type
    MOR-2662 Hi-Affi™ Recombinant Rabbit Anti-PER3 Monoclonal Antibody (DS2662AB) WB IgG

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