Rabbit Anti-Phospho-PTPN11 (Y542) Recombinant Antibody (clone 2E2) (CAT#: ZG-0533U)
Rabbit Anti-Phospho-PTPN11 (Y542) Antibody (clone 2E2) displayed high specificity against the PTPN11 protein of Human.
Specific Inquiry
WB
Figure 1 Rabbit Anti-Phospho-PTPN11 (Y542) Antibody (ZG-0533U) in WB
Western Blot
Positive WB detected in Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate(treated with Pervanadate or not)
All lanes Phospho-PTPN11 antibody at 0.65μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 68 KDa
Observed band size: 68 KDa
IP
Figure 2 Rabbit Anti-Phospho-PTPN11 (Y542) Antibody (ZG-0533U) in IP
Immunoprecipitating Phospho-PTPN11 in Hela whole cell lysate treated with Pervanadate
Lane 1: Rabbit control IgG(1μg)instead of ZG-0533U in Hela whole cell lysate treated with Pervanadate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0533U(3μg)+ Hela whole cell lysate treated with Pervanadate(1mg)
Lane 3: Hela whole cell lysate treated with Pervanadate(20μg)
Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-PTPN11 (Y542)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human Phospho-PTPN11 (Y542)
- Species Reactivity
- Human
- Clone
- 2E2
- Applications
- ELISA, WB, IP
- Conjugate
- Unconjugated
- Related Disease
- Noonan Syndrome 1 and Juvenile Myelomonocytic Leukemia.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunoprecipitation. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IP:1:200-1:1000
Target
- Alternative Names
- Tyrosine-protein phosphatase non-receptor type 11; Protein-tyrosine phosphatase 1D; PTP-1D; Protein-tyrosine phosphatase 2C; PTP-2C; SH-PTP2; SHP-2; Shp2; SH-PTP3; PTPN11; PTP2C; SHPTP2
- Gene ID
- 5781
- UniProt ID
- Q06124
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Chicken IgY Antibody Products
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0456MZ | Chicken Anti-PTPN11 Polyclonal IgY | WB | Chicken antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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