Rabbit Anti-Phospho-PXN (Y118) Recombinant Antibody (clone 1F11)
CAT#: ZG-0537U
Rabbit Anti-Phospho-PXN (Y118) Antibody (clone 1F11) displayed high specificity against the PXN protein of Human.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-Phospho-PXN (Y118) Antibody (ZG-0537U) in WB
Western Blot
Positive WB detected in Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate(treated with Calyculin A or EGF)
All lanes Phospho-PXN antibody at 1.43μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 68 KDa
Observed band size: 68 KDa
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & focal adhesion sites
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51309/795_G6_5_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3841/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3841/10426_A_9_3.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3841/10426_B_7_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Pachytene spermatocytes
Staining: Low
Intensity: Moderate
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3841/10426_A_4_6.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000089159-PXN/subcellular
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Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-PXN (Y118)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human Phospho-PXN (Y118)
- Species Reactivity
- Human
- Clone
- 1F11
- Applications
- ELISA, WB
- Conjugate
- Unconjugated
- Related Disease
- Neurofibromatosis, Type Ii and Breast Adenocarcinoma.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot. Specific conditions should be optimized by the end user.
WB:1:500-1:5000
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "PXN"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-314 | Human Anti-PXN Recombinant Antibody (clone CBL14Q) | WB, ELISA, IF, FuncS | Human IgG |
| PABL-674 | Human Anti-PXN Recombinant Antibody (clone CBL19Q) | WB, FuncS | Human IgG |
| PABL-675 | Human Anti-PXN Recombinant Antibody (clone CBL35Q) | WB, FuncS | Human IgG |
| FAMAB-1491CQ | Mouse Anti-PXN Recombinant Antibody (clone LD4 sAB) | WB, ELISA | Mouse IgG1 |
| FAMAB-1492CQ | Mouse Anti-PXN Recombinant Antibody (clone LD2 sAB) | WB, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-314 | Human Anti-PXN Recombinant Antibody (clone CBL14Q); scFv Fragment | WB, ELISA, IF, FuncS | Human scFv |
| PSBL-668 | Human Anti-PXN Recombinant Antibody (clone CBL19Q); scFv Fragment | WB, FuncS | Human scFv |
| PSBL-669 | Human Anti-PXN Recombinant Antibody (clone CBL35Q); scFv Fragment | WB, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-314 | Human Anti-PXN Recombinant Antibody (clone CBL14Q); Fab Fragment | WB, ELISA, IF, FuncS | Human Fab |
| PFBL-668 | Human Anti-PXN Recombinant Antibody (clone CBL19Q); Fab Fragment | WB, FuncS | Human Fab |
| PFBL-669 | Human Anti-PXN Recombinant Antibody (clone CBL35Q); Fab Fragment | WB, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2947 | Hi-Affi™ Recombinant Rabbit Anti-PXN Monoclonal Antibody (DS2947AB) | FC, ICC, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5893 | Anti-PXN Immunohistochemistry Kit | IHC | |
| VS-0525-XY5894 | Anti-Mouse PXN Immunohistochemistry Kit | IHC | |
| VS-0525-XY5895 | Anti-Rat PXN Immunohistochemistry Kit | IHC |
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