Rabbit Anti-PIAS1/2 Recombinant Antibody (clone R06-7C1)
CAT#: VS3-FY2106
This incredible rabbit recombinant monoclonal antibody is suitable for immunohistochemistry (IHC) validation purposes. It exhibits a specific binding affinity towards human PIAS1. The results of PIAS1/PIAS2 western blot analysis of mouse testis and rat testis lysates were positive.
Tested Data
WB
Figure 1 Anti-PIAS1/2 Antibody (VS3-FY2106) in WB.
The PIAS1/PIAS2 expression in mouse testis, rat testis lysates were detected using the anti-PIAS1/2 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide derived from human PIAS1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PIAS1/2
- Species Reactivity
- Human
- Clone
- R06-7C1
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 72 kDa, while the observed molecular weight stands at 76 kDa.
- Related Disease
- Nephronophthisis and Germ Cell Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Protein Inhibitor Of Activated STAT 1; DEAD/H (Asp-Glu-Ala-Asp/His) Box Binding Protein 1; Protein Inhibitor Of Activated STAT Protein 1; Zinc Finger, MIZ-Type Containing 3; RNA Helicase II-Binding Protein; DEAD/H Box-Binding Protein 1; Gu-Binding Protein; DDXBP1
- Gene ID
- 8554
- UniProt ID
- O75925
- Sequence Similarities
- Belongs to the PIAS family.
- Cellular Localization
- Cytoplasm, Cytoskeleton, Nucleus
- Post Translation Modifications
- Sumoylated. (O75925-PIAS1_HUMAN)
Ubiquitination at Lys8, Lys46, Lys339, and Lys493 (NX_O75925 [NX_O75925-1])
Modification sites at PhosphoSitePlus (O75925)
Glycosylation from GlyGen (O75925) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011520428.1; XP_011520429.1; XP_016878177.1; XP_016878178.1; XP_047289153.1
- Function
- Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB (By similarity).
Mediates the nuclear mobility and localization of MSX1 to the nuclear periphery, whereby MSX1 is brought into the proximity of target myoblast differentiation factor genes (By similarity).
Also required for the binding of MSX1 to the core enhancer region in target gene promoter regions, independent of its sumolyation activity (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
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