Rabbit Anti-PIAS1/2 Recombinant Antibody (clone R06-7C1) (CAT#: VS3-FY2106)

• Immunogen
• Synthetic peptide derived from human PIAS1

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human PIAS1/2

• Species Reactivity
• Human

• Clone
• R06-7C1

• Applications
• Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation

• MW
• The calculated weight of the target protein is approximately 72 kDa, while the observed molecular weight stands at 76 kDa.

• Related Disease
• Nephronophthisis and Germ Cell Cancer

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry: 1/50-1/100
  Immunoprecipitation: 1/20

• Alternative Names
• Protein Inhibitor Of Activated STAT 1; DEAD/H (Asp-Glu-Ala-Asp/His) Box Binding Protein 1; Protein Inhibitor Of Activated STAT Protein 1; Zinc Finger, MIZ-Type Containing 3; RNA Helicase II-Binding Protein; DEAD/H Box-Binding Protein 1; Gu-Binding Protein; DDXBP1

• Gene ID
• 8554

• UniProt ID
• O75925

• Sequence Similarities
• Belongs to the PIAS family.

• Cellular Localization
• Cytoplasm, Cytoskeleton, Nucleus

• Post Translation Modifications
• Sumoylated. (O75925-PIAS1_HUMAN)
  Ubiquitination at Lys8, Lys46, Lys339, and Lys493 (NX_O75925 [NX_O75925-1])
  Modification sites at PhosphoSitePlus (O75925)
  Glycosylation from GlyGen (O75925) 1 site, 1 O-linked glycan (1 site)

• Protein Refseq
• XP_011520428.1; XP_011520429.1; XP_016878177.1; XP_016878178.1; XP_047289153.1

• Function
• Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB (By similarity).
  Mediates the nuclear mobility and localization of MSX1 to the nuclear periphery, whereby MSX1 is brought into the proximity of target myoblast differentiation factor genes (By similarity).
  Also required for the binding of MSX1 to the core enhancer region in target gene promoter regions, independent of its sumolyation activity (By similarity).

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody