Rabbit Anti-PIK3CB Recombinant Antibody (clone 5A9) (CAT#: ZG-0512U)

Name: Rabbit Anti-PIK3CB Recombinant Antibody (clone 5A9)
SKU: ZG-0512U
Rating: 5 (1 reviews)

Rabbit Anti-PIK3CB Antibody (clone 5A9) displayed high specificity against the PIK3CB protein of Human.

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1 Rabbit Anti-PIK3CB Antibody (ZG-0512U) in WB

Figure 1 Rabbit Anti-PIK3CB Antibody (ZG-0512U) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate
All lanes: PIK3CB antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 123 kDa
Observed band size: 110 kDa

Figure 2 Rabbit Anti-PIK3CB Antibody (ZG-0512U) in IP

Figure 2 Rabbit Anti-PIK3CB Antibody (ZG-0512U) in IP

Immunoprecipitating PIK3CB in K562 whole cell lysate
Lane 1: Rabbit control IgG instead of ZG-0512U in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0512U(2µg)+ K562 whole cell lysate(500µg)
Lane 3: K562 whole cell lysate (10µg)

Subcellular Location

Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42288/1246_A2_1_selected.jpg

Figure 2 IF staining of human cell line SK-MEL-30
(Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64207/1269_F4_1_selected.jpg

Normal Tissue

Figure 3 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31938/ihc_selected.jpg

Figure 4 Cerebral cortex
(Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31938/80416_B_7_5.jpg

Figure 5 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31938/80416_A_8_3.jpg

Figure 6 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31938/80416_A_8_4.jpg

Figure 7 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31938/80416_A_8_5.jpg

Figure 8 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31938/80416_A_6_6.jpg

RNA Expression

Figure 9 RNA cell line category: Cell line enhanced (HEL, HMC-1)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000051382-PIK3CB

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
A synthesized peptide derived from human PI3 Kinase p110 beta

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human PIK3CB

Species Reactivity
Human

Clone
5A9

Applications
ELISA, WB, IP

Conjugate
Unconjugated

Related Disease
Nk-Cell Enteropathy and Immunodeficiency 14.

Product Property

Purification
Affinity chromatography purified

Buffer
PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Preservative
0.02% sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunoprecipitation. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IP:1:200-1:1000

Target

Alternative Names
Phosphatidylinositol 4;5-bisphosphate 3-kinase catalytic subunit beta isoform; PI3-kinase subunit beta; PI3K-beta; PI3Kbeta; PtdIns-3-kinase subunit beta; EC 2.7.1.153; Phosphatidylinositol 4;5-bisphosphate 3-kinase 110 kDa catalytic subunit beta; PtdIns-3-kinase subunit p110-beta; p110beta; PIK3CB; PIK3C1

Gene ID
5291

UniProt ID
P42338

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "PIK3CB"

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IgG Antibody Products Chicken IgY Antibody Products Rabbit Monoclonal Antibody Products

CAT	Product Name	Application	Type
MOB-0992MZ	Recombinant Mouse Anti-Human PIK3CB Antibody	IHC-P	Mouse antibody
MRO-1227-CN	Recombinant Rabbit Anti-PIK3CB Monoclonal Antibody (CBACN-448)	WB, IP, FC	Rabbit IgG
VS3-CJ953	Rabbit Anti-PIK3CB Recombinant Antibody (VS3-CJ953)	WB, IP, FC	Rabbit IgG
VS3-FY1139	Recombinant Rabbit Anti-PIK3CB Antibody (clone R03-5K7)	WB, IP	Rabbit IgG

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