Rabbit Anti-PIK3CB Recombinant Antibody (clone 5A9)
CAT#: ZG-0512U
Rabbit Anti-PIK3CB Antibody (clone 5A9) displayed high specificity against the PIK3CB protein of Human.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-PIK3CB Antibody (ZG-0512U) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate
All lanes: PIK3CB antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 123 kDa
Observed band size: 110 kDa
IP
Figure 2 Rabbit Anti-PIK3CB Antibody (ZG-0512U) in IP
Immunoprecipitating PIK3CB in K562 whole cell lysate
Lane 1: Rabbit control IgG instead of ZG-0512U in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0512U(2µg)+ K562 whole cell lysate(500µg)
Lane 3: K562 whole cell lysate (10µg)
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42288/1246_A2_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64207/1269_F4_1_selected.jpg
Normal Tissue
Figure 3 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31938/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31938/80416_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31938/80416_A_8_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31938/80416_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31938/80416_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31938/80416_A_6_6.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (HEL, HMC-1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000051382-PIK3CB
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Specifications
- Immunogen
- A synthesized peptide derived from human PI3 Kinase p110 beta
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PIK3CB
- Species Reactivity
- Human
- Clone
- 5A9
- Applications
- ELISA, WB, IP
- Conjugate
- Unconjugated
- Related Disease
- Nk-Cell Enteropathy and Immunodeficiency 14.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunoprecipitation. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IP:1:200-1:1000
Target
- Alternative Names
- Phosphatidylinositol 4;5-bisphosphate 3-kinase catalytic subunit beta isoform; PI3-kinase subunit beta; PI3K-beta; PI3Kbeta; PtdIns-3-kinase subunit beta; EC 2.7.1.153; Phosphatidylinositol 4;5-bisphosphate 3-kinase 110 kDa catalytic subunit beta; PtdIns-3-kinase subunit p110-beta; p110beta; PIK3CB; PIK3C1
- Gene ID
- 5291
- UniProt ID
- P42338
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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