Rabbit Anti-PIK3R1 Recombinant Antibody (VS3-FY2835) (CAT#: VS3-FY2835)
This anti-PIK3R1 antibody is a rabbit recombinant monoclonal antibody. It has been specifically designed to target the human PIK3R1 protein. It cross-reacts with rat, hamster species. The monoclonal antibody can be used in the area of Short Syndrome and Agammaglobulinemia 7, Autosomal Recessive. The antibody can be utilized for detection of PIK3R1 by Immunoprecipitation (1/20), Western Blot (1/500-1/1000) and Immunocytochemistry (1/50-1/200).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1216/28_C9_2_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1216/28_C9_2_selected.jpg
Figure 2 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in parietal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1216/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in parietal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1216/ihc_selected.jpg
Figure 3 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows strong positivity in a subset of glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4268/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows strong positivity in a subset of glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4268/ihc_selected.jpg
Figure 4 Cerebral cortex
(Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
Glial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
nuclear
Neuronal cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4268/19234_B_7_5.jpg
(Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
Glial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
nuclear
Neuronal cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4268/19234_B_7_5.jpg
Figure 5 Colon
(Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Peripheral nerve/ganglion
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4268/19234_A_7_3.jpg
(Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Peripheral nerve/ganglion
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4268/19234_A_7_3.jpg
Figure 6 Kidney
(Cells in glomeruli
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4268/19234_A_9_5.jpg
(Cells in glomeruli
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4268/19234_A_9_5.jpg
Figure 7 Testis
(Cells in seminiferous ducts
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Leydig cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4268/19234_A_6_6.jpg
(Cells in seminiferous ducts
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Leydig cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4268/19234_A_6_6.jpg
Figure 8 Breast
(Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Myoepithelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4268/19234_B_2_4.jpg
(Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Myoepithelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4268/19234_B_2_4.jpg
Figure 9 Lymph node
(Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4268/19234_A_9_8.jpg
(Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4268/19234_A_9_8.jpg
Figure 10 RNA cell line category: Cell line enhanced (ASC diff)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000145675-PIK3R1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000145675-PIK3R1
Figure 10 RNA cell line category: Cell line enhanced (ASC diff)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000145675-PIK3R1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000145675-PIK3R1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat and Hamster PIK3R1
- Species Reactivity
- Human, Rat, Hamster
- Applications
- Western Blot, Immunocytochemistry, Immunoprecipitation
- Conjugate
- Unconjugated, FITC
- Related Disease
- Short Syndrome and Agammaglobulinemia 7, Autosomal Recessive
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunoprecipitation: 1/20
Western Blot: 1/500-1/1000
Immunocytochemistry: 1/50-1/200
Target
- Alternative Names
- Phosphoinositide-3-Kinase Regulatory Subunit 1; Phosphatidylinositol 3-Kinase 85 KDa Regulatory Subunit Alpha; Phosphoinositide-3-Kinase, Regulatory Subunit 1 (Alpha); Phosphoinositide-3-Kinase Regulatory Subunit Alpha; PtdIns-3-Kinase Regulatory Subunit Alpha; PI3K Regulatory Subunit Alpha; PI3-Kinase Subunit P85-Alpha; GRB1; Phosphatidylinositol 3-Kinase, Regulatory Subunit, Polypeptide 1 (P85 Alpha)
- Gene ID
- 5295
- UniProt ID
- P27986
- Cellular Localization
- Cell-cell junction, Cis-Golgi network, Cytoplasm, Cytosol, Nucleus, Plasma membrane
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Please select product type
Rabbit Monoclonal Antibody Products
IgG Antibody Products
Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2711 | Hi-Affi™ Recombinant Rabbit Anti-PIK3R1 Monoclonal Antibody (DS2711AB) | WB, IHC, ICC, FC, IP | IgG |
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