Rabbit Anti-Pip5K1C Recombinant Antibody (clone R06-3J3)
CAT#: VS3-FY1834
Clone R06-3J3 is a rabbit recombinant monoclonal antibody that specifically directed against human Pip5K1C. It cross-reacts with mouse. It is suitable for use in a variety of assays, including WB, IP. Applications Tested: The PIP5K1C antibody has been tested by western blot analysis of PIP5KI gamma in mouse brain lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17168/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows cytoplasmic positivity in Purkinje cells, cells in molecular layer and cells in granular layer.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17168/40154_B_9_8_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17168/40154_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17168/40154_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17168/40154_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17168/40154_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17168/40154_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (U-2197)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000186111-PIP5K1C
❮
❯
WB
Figure 1 Anti-Pip5K1C Antibody (VS3-FY1834) in WB.
The PIP5KI gamma expression in mouse brain lysates were detected using the anti-PIP5K1C by western blot assay.
❮
❯
Specifications
- Immunogen
- Recombinant mouse PIP5KI γ protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse Pip5K1C
- Species Reactivity
- Human, Mouse
- Clone
- R06-3J3
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 72 kDa, while the observed molecular weight stands at 90,87 kDa.
- Related Disease
- Lethal Congenital Contracture Syndrome 3 and Lethal Congenital Contracture Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Phosphatidylinositol-4-Phosphate 5-Kinase Type 1 Gamma; Phosphatidylinositol-4-Phosphate 5-Kinase, Type I, Gamma; PtdIns(4)P-5-Kinase 1 Gamma; PIP5K1-Gamma; EC 2.7.1.68; Phosphatidylinositol 4-Phosphate 5-Kinase Type-1 Gamma; Phosphatidylinositol 4-Phosphate 5-Kinase Type I Gamma; Diphosphoinositide Kinase
- Gene ID
- 18717
- UniProt ID
- O70161
- Cellular Localization
- Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane
- Post Translation Modifications
- Phosphorylation on Ser-650 negatively regulates binding to TLN2 and is strongly stimulated in mitosis.
Phosphorylation on Tyr-649 is necessary for targeting to focal adhesions.
- Protein Refseq
- XP_011526150.1; XP_011526151.1; XP_016882029.1; XP_047294489.1; XP_047294490.1
- Function
- PtdIns(4,5)P2 can directly act as a second messenger or can be utilized as a precursor to generate other second messengers: inositol 1,4,5-trisphosphate (IP3), diacylglycerol (DAG) or phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3/PIP3) (By similarity).
PIP5K1A-mediated phosphorylation of PtdIns4P is the predominant pathway for PtdIns(4,5)P2 synthesis (By similarity).
Plays a role in endocytosis mediated by clathrin and AP-2 (adaptor protein complex 2).
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY1834. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PIP5K1C"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1605CT | Recombinant Mouse anti-Human PIP5K1C Monoclonal antibody (EML1359) | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2720 | Hi-Affi™ Recombinant Rabbit Anti-PIP5K1C Monoclonal Antibody (DS2720AB) | WB, IP | IgG |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
-3.png)
Application: ELISA, FC, Block, SPR
Application: ELISA, FC
Application: ELISA, FuncS
Application: ELISA, WB, Microarray, Block
Application: ELISA, IHC, IF, IP
Application: EM, ELISA, ICC, IHC-Fr, IHC-P, WB
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
